关键词: 菠萝蜜, 高效液相色谱, 葡萄糖, 果糖, 蔗糖

Abstract:

Sugars including glucose, fructose and sucrose in different fruit tissues of jackfruit were extracted by 3 different
extraction methods and determined by high performance liquid chromatography (HPLC). The HPLC conditions were set as
follows: the chromatograph was equipped with a CHO-820(Ca) column (300 mm × 7.8 mm) and a refractive index detector;
ultra-pure water was used as eluent with a flow rate of 0.5 mL/min; the column temperature was 90 ℃. Results showed
that 100% ethenol was the optimum extraction solvent for sucrose while 80% ethanol was the best solvent for simultaneous
extraction of glucose, fructose and sucrose. The relative standard deviations were in the range from 1.51% to 7.01%, and
the recoveries for spiked samples ranged from 98.55% to 102.33% and the correlation coefficients were higher than 0.999 0
for linearity, showing that the method is effective to isolate the three sugars from jackfuit. Results also showed that jackfruit
pulp contained 14.01 g/100 g mf sugars on a fresh weight basis with sucrose being the main component while the seed coat
contained 10.19 g/100 g mf, with fructose and glucose being the main components and Guojian (developed from unfertilized
or partly fertilized female perianth tissues) contained only 3.54 g/100 g mf. The fructose contents were similar to sucrose
contents in the 3 different tissues.

Key words: jackfruit, high performance liquid chromatography (HPLC), glucose, fructose, sucrose