高效液相色谱法定量测定果蔬中甲醛含量

doi:10.7506/spkx1002-6630-201622030

食品科学 ›› 2016, Vol. 37 ›› Issue (22): 198-204.doi: 10.7506/spkx1002-6630-201622030

高效液相色谱法定量测定果蔬中甲醛含量

李俊¹,²，刘永翔²，王辉¹,²，刘嘉¹,²，刘辉²，阚建全¹,*

1.西南大学食品科学学院，重庆 400715；
2.贵州省生物技术研究所，贵州贵阳 550006

收稿日期:2016-04-10 出版日期:2016-11-16 发布日期:2017-02-22
通讯作者: 阚建全（1965—），男，教授，博士，研究方向为食品化学与营养学。E-mail：kanjianquan@163.com
作者简介:李俊（1990—），男，研究实习员，硕士，研究方向为食品质量与安全。E-mail：lijunsjs2015@163.com
基金资助:
中华人民共和国农业部财政专项（GJFP201501201）

Determination of Formaldehyde in Fruits and Vegetables by HPLC

LI Jun¹,², LIU Yongxiang², WANG Hui¹,², LIU Jia¹,², LIU Hui², KAN Jianquan¹,*

1. College of Food Science, Southwest University, Chongqing 400715, China；
2. Biological Technology Institute of Guizhou Province, Guiyang 550006, China

Received:2016-04-10 Online:2016-11-16 Published:2017-02-22

摘要/Abstract

摘要： 通过五氟苯肼与甲醛进行衍生，建立高效液相色谱法定量测定果蔬中甲醛含量的分析方法。样品在温度为60 ℃超声波条件下直接提取衍生30 min，离心纯化后高效液相色谱检测，外标法定量。优化的色谱条件为：XDB-C18色谱柱（4.6 mm×250 mm，5 μm），流动相：乙腈-水（70∶30，V/V），流速1.0 mL/min，柱温40 ℃，检测波长258 nm。结果表明，该方法的定量检测限可达到0.114 mg/kg，在0.114～57 mg/L范围内呈良好的线性关系，当甲醛添加量分别为1.14、8.55、17.10 mg/kg时，平均回收率为81.4%～101.8%，相对标准偏差为2.6%～5.0%（n=6）。该方法样品前处理简便、稳定性好、检测限低，适合果蔬中甲醛含量的快速检测。

关键词: 果蔬, 甲醛, 高效液相色谱, 五氟苯肼

Abstract: A high performance liquid chromatography (HPLC) method was used to determine formaldehyde in fruits and vegetables by formaldehyde derivation with pentafluorophenylhydrazine (PFPH). Samples were extracted and derived at 70 ℃ with ultrasonicaction for 30 min. After completion of the reaction, centrifugation was carried out and the supernatant was purified and analyzed by HPLC. The quantitation was performed using the external standard method. The separation was performed on an XDB-C18 column (4.6 mm × 250 mm, 5 μm) in an isocratic elution mode using a mobile phase consisting of acetonitrile and water (70:30, V/V) at a flow rate of 1.0 mL/min. The column temperature was 40 ℃ and detection wavelength was 258 nm. The results showed that the limit of quantification (LOQ) of the presented method was 0.114 mg/kg, with a good linear relationship in the range of 0.114–57 mg/L. The average recovery rates at three spiked levels were 81.4%–101.8%, with relative standard derivation (RSD) of 2.6%–5.0% (n = 6). The method was simple, stable and suitable for the determination of formaldehyde in fruits and vegetables with a low limit of detection (LOD).

Key words: fruits and vegetables, formaldehyde, HPLC, pentafluorophenylhydrazine

中图分类号:

TS255.7

李俊,刘永翔,王辉,刘嘉,刘辉,阚建全,*. 高效液相色谱法定量测定果蔬中甲醛含量[J]. 食品科学, 2016, 37(22): 198-204.

LI Jun, LIU Yongxiang, WANG Hui, LIU Jia, LIU Hui, KAN Jianquan,*. Determination of Formaldehyde in Fruits and Vegetables by HPLC[J]. FOOD SCIENCE, 2016, 37(22): 198-204.

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高效液相色谱法定量测定果蔬中甲醛含量

Determination of Formaldehyde in Fruits and Vegetables by HPLC

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