芜菁酸性多糖结构及对LPS诱导肺损伤小鼠的保护作用

摘要/Abstract

摘要： 摘要目的：研究芜菁酸性多糖（Brassica rapa L. Acidic Polysachharide-1，BRAP-1）结构及对脂多糖（LPS）诱导的小鼠肺损伤的影响及其机制。方法：采用苯酚硫酸法、间羟基联苯法、考马斯亮蓝法、高效凝胶渗透色谱、FT-IR、GC-MS法对其化学组成、分子量、结构及单糖组成进行分析。正常组、模型组灌胃0.5ml·d-1生理盐水，BRAP-1低、中、高剂量组分别灌胃50、100、200 mg·kg-1·d-1，持续10天，采用LPS气管滴注法建立肺损伤模型。检测相关指标：肺湿重/干重比值；肺泡灌洗液蛋白含量、总细胞数；HE染色；ELISA法测定各组小鼠血清中TNF-α、IL-6、IL-10、IFN-γ细胞因子的含量；qRT-PCR法检测肺组织中TNFR、TLR4、P-65、IL-1β、NLRP3基因mRNA表达水平。结果：与模型组相比较，BRAP-1对肺损伤小鼠肺组织水肿、肺泡浸润、蛋白渗出、细胞因子过度释放等病理学改变有缓解作用；并显著降低NF-κB/NLRP3通路关键基因mRNA表达水平。结论：BRAP-1对LPS诱导的小鼠肺损伤具有显著保护作用，其机制可能是调节NF-κB/NLRP3信号通路。

关键词: 芜菁, 酸性多糖, 肺损伤, 保护作用, NF-κB/NLRP3信号通路

Abstract: Abstract Objective: To study the structure of Brassica rapa L. acidic polysachharide-1 (BRAP-1) and its effect and mechanism on Lipopolysaccharide (LPS) -induced lung injury in mice. Methods: The chemical composition, molecular weight, structure and monosaccharide composition were analyzed by phenol-sulfuric acid method, m-hydroxybiphenyl method, Coomassie brilliant blue method, high performance gel permeation chromatography, FT-IR and GC-MS. Control group and model group mice were gavaged with 0.5 ml/d PBS, BRAP-1 groups mice were gavaged with 50, 100 and 200 mg·kg-1·d-1 for 10 days. The model was established by LPS tracheal infusion method. Test related indicators: lung wet weight/dry weight ratio;Broncho alveolar lavage fluid(BALF) protein concentration,total number of cells;HE staining; ELISA was used to determine the TNF-α、IL-6、IL-10、IFN-γ cytokines concentration in serum of mice in each group;qRT-PCR was used to detect the mRNA expression of TNFR, TLR4, P-65, IL-1β and NLRP3 in lung tissue. Results: Compared with the model group, BRAP-1 can alleviate the pathological changes of lung tissue edema, alveolar infiltration, protein exudation and excessive cytokines release in mice with lung injury ,Significantly decreased the mRNA expression levels of key genes in NF-KB/NLRP3 pathway. Conclusion: BRAP-1 has a significant protective effect on LPS induced lung injury in mice,the mechanism may be the regulation of NF-κB/NLRP3 signaling pathway.

Key words: Brassica rapa L., acidic polysachharide, lung injury, protective effect, NF-κB/NLRP3 pathway

中图分类号:

TS201.4

卡地尔亚·库尔班海力茜·陶尔大洪杨飞. 芜菁酸性多糖结构及对LPS诱导肺损伤小鼠的保护作用[J]. 食品科学, 0, (): 0-0.

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