食品科学 ›› 2025, Vol. 46 ›› Issue (17): 292-266.doi: 10.7506/spkx1002-6630-20250331-239

• 安全检测 • 上一篇    

纳米超表面等离子体共振芯片超灵敏检测水产品中氯霉素

冀威昊,龚蕾,梁嘉宁,周陶鸿,范小龙,陈锂   

  1. (1.湖北省食品质量安全监督检验研究院,国家市场监管重点实验室(动物源性食品中重点化学危害物检测技术),湖北?武汉 430075;2.湖北江城实验室,湖北?武汉 430205)
  • 发布日期:2025-08-18
  • 基金资助:
    国家市场监督管理总局科技计划项目(2023MK087);湖北省市场监管局技术保障专项(Hbscjg-JS2021009)

Ultrasensitive Immunoassay Based on Meta-surface Plasma Resonance Chip for Chloramphenicol in Aquatic Products

JI Weihao, GONG Lei, LIANG Jianing, ZHOU Taohong, FAN Xiaolong, CHEN Li   

  1. (1. Key Laboratory of Detection Technology of Focus Chemical Hazards in Animal-Derived Food for State Market Regulation, Hubei Provincial Institute for Food Supervision and Test, Wuhan 430075, China; 2. Yangtze Laboratory, Wuhan 430205, China)
  • Published:2025-08-18

摘要: 基于纳米杯阵列结构的超表面等离子体共振芯片,结合金纳米颗粒(Au nanoparticles,AuNPs),构建水产品中氯霉素(chloramphenicol,CAP)的免疫竞争传感平台。通过扫描电镜、纳米粒度分析仪等手段表征芯片及AuNPs的结构、粒径等性质;通过不同条件下亲和力的测试探究免疫竞争传感策略的可行性;在优化条件下,传感器对CAP的检出限为0.06 ng/mL,检测范围为0.125~16 ng/mL(R2=0.998),加标回收率为102.5%~105.0%,具有良好的稳定性和特异性。此外,本检测方法速度快(10 min),通量高(96 个检测孔同时检测),在食品安全快速检测方面有很高的应用价值。

关键词: 纳米超表面等离子体共振芯片;金纳米颗粒;氯霉素;水产品

Abstract: Meta-surface plasmon resonance (MetaSPR) chip and gold nanoparticles (AuNPs) were employed to construct an immunocompetitive sensing platform for detecting chloramphenicol (CAP) in aquatic products. The structure, particle size and other properties of the chip and AuNPs were characterized using scanning electron microscopy (SEM) and a nanoparticle size analyzer. Binding affinities in varying conditions were tested to verify the feasibility of the immunocompetitive sensing strategy. Under optimized conditions, the detection limit of the sensor was 0.06 ng/mL, with a linear detection range of 0.125–16 ng/mL (R2 = 0.998). The spiked recovery rates of CAP in aquatic products ranged from 102.5% to 105.0%, indicating excellent stability and specificity. Furthermore, the sensor was rapid (10 min) and high-throughput (simultaneous detection in 96 wells), demonstrating significant potential in food safety monitoring.

Key words: meta-surface plasmon resonance chip; gold nanoparticles; chloramphenicol; aquatic products

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