HPLC Determination of Aspartame and Its Metabolite in Commercial Beverages

doi:10.7506/spkx1002-6300-200918054

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (18 ): 249-251.doi: 10.7506/spkx1002-6300-200918054

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HPLC Determination of Aspartame and Its Metabolite in Commercial Beverages

LI Hao1，SONG Yang-cheng1，TENG Hong-gang2，CHEN Yu-fei3，ZHOU Quan1，CHEN Chang-wu1

1. Jilin Teacher＇s Institute of Engineering and Technology, Changchun 130052, China；2. Jilin Yatai Biopharmaceuticals Co. Ltd.,
Changchun 130032, China；3. Jilin Business and Technology College, Changchun 130062, China

Received:2009-06-10 Online:2009-09-15 Published:2010-12-29
Contact: LI Hao E-mail:muyelee@sina.com

Abstract

Abstract:

A HPLC method was presented for the rapid determination of aspartame and its metabolite in commercial beverages. The analytes were separated on a ZORBAX SB-C18 column using a mobile phase composed of 0.1% trifluoroacetic acid and acetonitrile (85:15, V/V) and detected using a DAD detector at 210 wavelength. The limits of detection of phenylalanine and aspartame by the method were 0.8 and 2.4 ng, respectively. There was a good linear relationship between concentration and peak area, and the correlation coefficients were 0.9998 and 0.9994, respectively. The spiked recoveries were 96.3% and 98.5%, respectively, with a RSD less than 2%. The method is simple, rapid and highly sensitive.

Key words: beverage, aspartame, phenylalanine, HPLC

CLC Number:

TS207.3

LI Hao1，SONG Yang-cheng1，TENG Hong-gang2，CHEN Yu-fei3，ZHOU Quan1，CHEN Chang-wu1. HPLC Determination of Aspartame and Its Metabolite in Commercial Beverages[J]. FOOD SCIENCE, 2009, 30(18 ): 249-251.

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HPLC Determination of Aspartame and Its Metabolite in Commercial Beverages

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