FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (24): 324-326.doi: 10.7506/spkx1002-6630-200924071

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Use of Pre-column Derivatization HPLC for Determination of Monosaccharide Compositions and Contents in Sorbus tianschanica Ruper. Polysaccharide

MENG Lei,CHANG Jun-min*,SUN Lian,XUE Zhao-yun   

  1. College of Pharmacy, Xinjiang Medical University, Urumqi 830011, China
  • Received:2008-12-17 Online:2009-12-15 Published:2010-12-29
  • Contact: CHANG Jun-min*, E-mail:cjmcjn2471@yahoo.com.cn

Abstract:

Objective: To determine the monosaccharide compositions in polysaccharide from Sorbus tianschanica Ruper.. Methods: Sorbus tianschanica Ruper. polysaccharide samples were hydrolyzed with 2 mol/L sulfuric acid solution, and derivatized with 1- phenyl-3 - methyl -5 - pyrazolone (PMP), and further separated by RP-HPLC using a equivalent elution process detected at 245 nm. Results: Polysaccharide of Sorbus tianschanica Ruper. was composed of mannose, rhamnose, galacturonic acid, glucuronide acid, glucose, galactose, arabinose. Conclusion: The method was simple, fast and reproducible, and can be used in determination of polysaccharide compositions of Sorbus tianschanica Ruper..

Key words: HPLC, Sorbus tianschanica Ruper. polysaccharides, pre-column derivatization, monosaccharide

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