Protoplast Fusion between Cordyceps sinensis and Cordyceps militaris

doi:10.7506/spkx1002-6630-201001039

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (1): 165-171.doi: 10.7506/spkx1002-6630-201001039

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Protoplast Fusion between Cordyceps sinensis and Cordyceps militaris

GUO Cheng-jin1，ZHAO Run2，ZHU Wen-bi3

(1. College of Chemistry and Life Science, Tianjin Normal University, Tianjin Key Laboratory of Cyto-Genetical and Molecular
Regulation, Tianjin 300387, China；2. Place of Scientific Research and Monitoring on Agricultural Environmental Protection,
Tianjin 300191, China；3. Dean's Office, Tianjin Agricultural University, Tianjin 300384, China)

Received:2009-03-25 Online:2010-01-01 Published:2014-05-19
Contact: GUO Cheng-jin1， E-mail:chengjin@vip.sina.com

Abstract

Abstract:

Fusion between protoplasts from Cordyceps sinensis and Cordyceps militaris subjected to heat and ultraviolet inactivations, respectively, was studied. In order to maximize fusion rate, five crucial fusion conditions were optimized using orthogonal array design. A maximum fusion rate of 2.74 × 10-5 was achieved through 20 min fusion at 35 ℃ under the promotion of 40% PEG and 0.08 mmol/L CaCl2 (pH 7). Besides, the genetic relationships among the parent and fusant strains were quested by morphologic observation, rivalry experiments and RAPD-PCR identification.

Key words: Cordyceps sinensis, Cordyceps militaris, fusant, genetic relationship

CLC Number:

Q949.3

GUO Cheng-jin1，ZHAO Run2，ZHU Wen-bi3. Protoplast Fusion between Cordyceps sinensis and Cordyceps militaris[J]. FOOD SCIENCE, 2010, 31(1): 165-171.

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Protoplast Fusion between Cordyceps sinensis and Cordyceps militaris

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