Extraction, Purification and Bile Acid-binding Capacity in vitro of Polysaccharides from Okra

doi:10.7506/spkx1002-6630-201013026

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (13): 110-113.doi: 10.7506/spkx1002-6630-201013026

• Basic Research • Previous Articles Next Articles

Extraction, Purification and Bile Acid-binding Capacity in vitro of Polysaccharides from Okra

REN Dan-dan CHEN Gu*

College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China

Received:2009-09-21 Online:2010-07-01 Published:2010-12-29
Contact: CHEN Gu E-mail:chengu@scut.edu.cn

Abstract

Abstract:

In order to investigate active components having the ability to bind bile acid from okra, Abelmoschus esculentus (L.) Moench, raw polysaccharide (RPS) was obtained from the fresh fruits of okra by water extraction and ethanol precipitation. RPS was further purified by DEAE anion exchange chromatography to obtain fractions E1, E2 and E3. Their bile acid-binding capacities in vitro were determined. The results indicated that fractions E1 and E2 presented different bile acid binding capacities, which accounted for 10.82% and 10.60% of that of cholestyramine, respectively. These investigations provide some significant references for exploring and developing okra as health food or functional additives.

Key words: okra, polysaccharide, ethanol precipitation, anion exchange chromatography, bile acid binding capacity

CLC Number:

REN Dan-dan CHEN Gu*. Extraction, Purification and Bile Acid-binding Capacity in vitro of Polysaccharides from Okra[J]. FOOD SCIENCE, 2010, 31(13): 110-113.

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Extraction, Purification and Bile Acid-binding Capacity in vitro of Polysaccharides from Okra

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