Abstract:

Objective: To explore the antioxidant effect of wheat germ bioactive peptides (WGBP) in aging mice induced by D-galactose. Methods: Fifty male Kunming mice were randomly divided into five groups, control group, model group, and WGBP low-, medium- and high-dose groups. Except the control group, all the other groups were subjected to subcutaneous injection of D-galactose solution at a dose of 600 mg/(kg bw·d). Mice were treated with WGBP at doses of 200, 800 mg/(kg bw·d) and 1000 mg/(kg bw·d) once a day for the creation of the WGBP low-, medium- and highdose groups. The activities of SOD and GSH-Px, T-AOC and the content of MDA in serum, brain, liver and heart were determined after a trial period of 45 days. Results: The activities of GSH-Px, SOD and T-AOC exhibited a significant increase (P ＜ 0.01) in serum, and the activities of SOD and T-AOC also exhibited a significant increase in heart (P ＜ 0.01) due to WGBP treatment. The activity of GSH-Px in brain and liver exhibited an obvious increase (P ＜ 0.05); meanwhile, an obvious increase of SOD activity in brain was also observed (P ＜ 0.05). Furthermore, the content of MDA in serum and the above organs revealed a reducing trend (P ＜ 0.05). Conclusion: WGBP has good antioxidant effect in vivo as expected.

Key words: wheat germ bioactive peptides, D-galactose, mice, antioxidant effect, in vivo