Separation and Purification of Cordycepin from Spent Solid Culture Medium after Harvesting Cordyceps militaris Fruit Bodies by Macroporous Resin Adsorption

doi:10.7506/spkx1002-6630-201206024

FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (6): 110-114.doi: 10.7506/spkx1002-6630-201206024

• Processing Technology • Previous Articles Next Articles

Separation and Purification of Cordycepin from Spent Solid Culture Medium after Harvesting Cordyceps militaris Fruit Bodies by Macroporous Resin Adsorption

HU Xia1,2，XIE Hong-qi1，LUO Wei1，XIA Zhi-lan1，LIU Dong-bo1,*

(1. Sub-health Intervention Technology Laboratory, State Administration of Traditional Chinese Medicine, College of Horticulture and Landscape, Hunan Agricultural University, Changsha 410128, China； 2. Changsha Subcenter of National Center of Citrus Improvement, Changsha 410128, China )

Online:2012-03-25 Published:2012-03-03

Abstract

Abstract: The adsorption and desorption characteristics of macroporous resins D101, AB-8, HPD-100, HPD-400, HPD-500, HPD-722 and DM130 for cordycepin from spent solid culture medium after harvesting Cordyceps militaris fruit bodies were comparatively investigated. The results showed that HPD-100 was the best resin for the purification of cordycepin from the spent culture medium. The optimal separation and purification conditions were cordycepin concentration of 0.6 mg/mL, sample loading amount of 6 BV, sample loading flow rate of 3 BV/h, 4 BV of 25% ethanol as desorbent, and desorption flow rate of 2 BV/h. Under these conditions, the purity of cordycepin could reach up to 14.1%, which was 8 times higher than before purification.

Key words: cordycepin, Cordyceps militaris, macroporous resin

CLC Number:

R284.2

HUXia，XIEHong-qi，LUOWei，XIAZhi-lan，LIUDong-bo. Separation and Purification of Cordycepin from Spent Solid Culture Medium after Harvesting Cordyceps militaris Fruit Bodies by Macroporous Resin Adsorption[J]. FOOD SCIENCE, 2012, 33(6): 110-114.

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Separation and Purification of Cordycepin from Spent Solid Culture Medium after Harvesting Cordyceps militaris Fruit Bodies by Macroporous Resin Adsorption

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