Abstract: In this study, one-factor-at-a-time and orthogonal array design methods were employed to optimize the hot water bath extraction of crude polysaccharide from young stems of Oenanthe javanica. Crude polysaccharide was deprotenized by Sevag method and sequentianlly precipitated with 1,3-fold and 6-fold volumes of absolute ethanol. All precipited fractions were anlyzed by GC-MS for monosaccharide composition and by atomic absorption spectrometry for partial element composition. The optimal conditons for crude polysaccharide extraction conditions were determined as 100 ℃ of temperature, 1:40 of material-to-liquid ratio (g/mL), and 3 h of extraction time. Under these conditons, the extraction yield of crude polysaccharide was 16.89%. Crude polysaccharide from young stems of Oenanthe javanica consisted of five hexoses such as D-glucose, rhamnose, idose, D-mannose, D-galactose and 7 hexose derivatives such as 1-methyl-α-rhamnoside, methyl-β-D-arab pyran glucoside, methyl-α-1-tallo glucoside, methyl glucoside, α-D-methyl-mannosidase, 1-methyl-α- rham- noside and glycuronic acid. The chactercteristic elements were Zn and Mn. The Zn and Se contents of the precipitate obtained with 1-fold vlome of absolute ethanol were 650.625 μg/g and 4.483 μg/g, respectivley. The Mn content of the precipitate obtaiend with 6-fold volume of absolute ethanol was 725.875 μg/g. In conclusion, crude polysaccharide from young stems of Oenanthe javanica can be developed as Zn and Se supplements.

Key words: Oenanthe javanica, polysaccharide, extraction process, monosaccharide composition