Anti-hyperglycemic Activities of Exopolysaccharides from Cordyceps militaris in Diabetic Mice Induced by Multiple Low-dose Streptozotocoin

doi:10.7506/spkx1002-6630-201311062

Abstract

Abstract:

Cordyceps militaris, a Chinese herbal medicine with fruiting body or cultured mycelia, has been proposed
to have multiple medicinal activities. The aim of this study is to investigate the hypoglycemic activity of the crude
polysaccharides, extracted from fruiting body (BP), cultured mycelia(MP) and exopolysaccaride(EP), in diabetic mice
induced by multiple low-dose streptozotocin (MLD-STZ). Only EP showed potent hypoglycemic activity in diabetic
mice during the observing period, and this treatment group was examined immunologically. The proliferative response
of the spleen cells stimulated with Concanavalin A(ConA), the IL-4 and IFN-γ levels of the supernatant in stimulated
splenocytes, and the IL-6 and TNF-α levels secreted from the spleen macrophage stimulated with LPS were compared
among the control group, the EP group and the untreated diabetic group. Meanwhile, the proliferative response of the
lymphocytes and the related cytokines levels were also investigated with different concentrations of EP in vitro. Our
results showed that both the blastogenesis response and cytokines levels (IL-4 and IFN-γ) of splenocytes stimulated
by ConA were decreased in EP group, meanwhile the IFN-γ/IL-4 ratio was moderately inhibited. IL-6 secretion of the
splenic macrophage of the EP group was also significantly enhanced leading to a lower TNF-α/IL-6 ratio than that of the
diabetic mice. These data suggest that EP treatment could reduce the blood glucose in MLD-STZ induced diabetic mice.
The immunological basis of the hypoglycemic activity was probably associated with inhibiting the lymphocytes response
and inducing the differentiation of Th cells into Th2 cells through enhancement of IL-6 production by the splenic
macrophages.

Key words: Cordyceps militraris, cytokines, polysaccharide, type 1 diabetes

CLC Number:

SUN Na-xin，YANG Gui-wen，AN Li-guo. Anti-hyperglycemic Activities of Exopolysaccharides from Cordyceps militaris in Diabetic Mice Induced by Multiple Low-dose Streptozotocoin[J]. FOOD SCIENCE, 2013, 34(11): 288-295.

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