Abstract: In order to validate the anti-aging effect of Bama longevity dietary patterns, a mouse model of aging was established by subcutaneously injecting D-galactose (500 mg/(kg·d)) into animals once daily for eight consecutive weeks. Meanwhile, the representative nutrients in Bama longevity dietary patterns (including calorie restriction and intake of VA and soybean isoflavones, dietary fiber and trace elements such as iron, manganese, cobalt and selenium) were selected to design five characteristic diets, calorie restriction, VA and soybean isoflavones, dietary fiber, trace elements and compound diet, for feeding aging mice. The contents of malondialdehyde (MDA) in serum, liver and brain, the activity of total superoxide dismutase (T-SOD) in serum and brain, and total antioxidant capacity (T-AOC) in liver and brain were measured. The results showed that all five diets could inhibit the accumulation of MDA in aging mice, while the activity of antioxidant enzymes and T-AOC were improved when compared with the aging model group. Through positive processing and nondimensionalization, it was found that compound nutrition had the best anti-oxidative stress effect , which could inhibit the accumulation of MDA in serum, liver and brain (P < 0.05), increase the activity of T-SOD by 12.33% (P < 0.05), and significantly increase the activity of T-AOC in liver by 35.35% (P < 0.05). A comprehensive evaluation showed that the antiaging effect of 5 diets followed the decreasing order of compound diet > VA and soybean isoflavones > calorie restriction > dietary fiber > trace elements. These results demonstrate that the Bama longevity diet pattern has a significant protective effect against oxidative stress in D-galactose-induced aging mice, indicating its great development potential.

Key words: Bama longevity dietary patterns, anti-oxidative stress effect, D-galactose, aging model