Isolation of Water-soluble Polysaccharides from Semen Cassiae

Abstract

Abstract: Acrude water -soluble polysaccharide(CP 1 )was extracted fromfried Semen Cassiae by hot water.It wasdividedintotwofractionsC 1 AandC 1 CbyFehling’ ssolution.Inthesameway,CP 2 andCP 3 wereextractedfrom freshSemenCassiaeandleaves ofSem enCassiae,respectively.Eachfractionwas separatedbyDEAE -cellulose column.Theresults showedthatC 1 Awas aneutralandrelativelypure polysaccharide,whereas C 1 Cwas acomplex polysaccharide.Therewas nosignif icantdifferencebetweentheDEAE -c ellulosechromatographiceluatecu rves of both CP 1 and CP 2 ,whose main fraction was a neutral polysaccharide.The main fraction of D EAE -cellulose chromatography of CP 3 was an acidic polysaccharide,which was quite different fromthe others.

Key words: semen, cassiae, polysaccharide, is olation

LIU Juan, DENG Ze-Yuan, LIU Jing-Jing, WANG San. Isolation of Water-soluble Polysaccharides from Semen Cassiae[J]. FOOD SCIENCE, 2003, 24(1): 67-69.

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Isolation of Water-soluble Polysaccharides from Semen Cassiae

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