Application of Placket-Burman Design for Determining Key Factors on Mycelium Growth and Extracellular Polysaccharides Excretion of Ganoderma lucidum

Abstract

Abstract: The Plackett-Burnam design was applied to screen out the key factors from the following eleven components, namely, glucose, sucrose, corn syrup powder, peptone, soybean bud juice, KH2PO4, Na2HPO4, MgSO4, ZnSO4•7H2O, VB1 and sodium glutamate, which would affect the mycelium growth and extracellular polysaccharides excretion of Ganoderma lucidum. With JMP software(version 4.0.5, SAS Institute Inc, USA)statistical analysis was performed. It was found that sucrose (p=0.0119), KH2PO4 (p=0.0111) and ZnSO4 (p=0.0024) were significant factors for mycelium growth; sucrose(p=0.0165), KH2PO4 (p=0.0103) and ZnSO4 (p=0.0019) were significant factors for extracellular polysaccharides excretion of Ganoderma lucidum.

Key words: Plackett-Burman design, Ganoderma lucidum, polysaccharide, submerged fermentation

CHEN Zhi-Jie, HAN Yong-Bin, SHEN Chang, CHEN De-Ming, GU Zhen-Xin. Application of Placket-Burman Design for Determining Key Factors on Mycelium Growth and Extracellular Polysaccharides Excretion of Ganoderma lucidum[J]. FOOD SCIENCE, 2005, 26(12): 115-118.

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Application of Placket-Burman Design for Determining Key Factors on Mycelium Growth and Extracellular Polysaccharides Excretion of Ganoderma lucidum

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