Application of Genome Shuffling in Enhancing L-lactic Acid Production by Improving Glucose Tolerance of Lactobacillus rhamnosus

Abstract

Abstract: In this paper,application of the genome shuffling in improving the glucose tolerance of Lactobacillus rhamnosus MEE539 and enhance the L-lactic acid production was studied.The starting population was generated by ultraviolet irradiation and nitrosoguanidine mutagenesis.The effect of amino acid and mutanolysin on protoplast formation was investigated.The positive colonies from library created by inactivated protoplasts were more likely to be screened on plates containing different concentrations of high glucose and 2% CaCO3.Characterization of all mutants and wide-type strain in shake flask indicated the compatibility of two optimal phenotypes of glucose tolerance and lactic acid enhancement.The lactic acid production of F2-2 from the second round genome shuffled population was 1.8 fold higher than that of the wild type at the initial glucose concentration of 150 g/L in 16l bioreactor.

Key words: genome shuffling, Lactobacillus rhamnosus, glucose tolerance, L-lactic acid

YU Lei, LEI Ting, PEI Xiao-Lin, LIU Jing-Sheng. Application of Genome Shuffling in Enhancing L-lactic Acid Production by Improving Glucose Tolerance of Lactobacillus rhamnosus[J]. FOOD SCIENCE, 2007, 28(9): 369-373.

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Application of Genome Shuffling in Enhancing L-lactic Acid Production by Improving Glucose Tolerance of Lactobacillus rhamnosus

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