Abstract: Panax ginseng and Daucus carota plant tissues were co-cultured by suspension way, and the best medium for suspension co-culture was screened in this study. Meanwhile metabolites of Panax ginseng and Daucus carota and their co-culture plant tissues were assayed respectively. The results indicated the best medium for supension co-culture with Daucus carota and Daucus carota is MS, 2,4-D 2 mg/L, NAA 1 mg/L and KT 0.2 mg/L, after 25 d the polysaccharide content of co-culture reaches the highest level 0.203 mg/g, and the saponin content of co-culture is 1.85 mg/g, higher 3.35% than saponin of Panax ginseng culture.

Key words: Panax ginseng, Daucus carota, suspension co-culture, Panax saponin, polysaccharose