An Efficient and Rapid Method for DNA Extraction and Species Identification from Sea Cucumber Nutrient Solution

Abstract

Abstract: Mitochondria DNA of sea cucumber were extracted from the sea cucumber nutrient solution with optimized method.The quantitative and qualitative analyses of DNA solution were performed with the calculation of A260/A280,Agarose gel electrophoresis,and PCR.The results showed that our method of DNA extraction and species identification from the sea cucumber nutrient solution was more efficient and rapid than traditional methods.This study will be helpful for quicker,more economical,and dependable species identification of the sea cucumber.

Key words: sea cucumber nutrient solution, DNA extraction, species identification

LIANG Jun-Ni, XUE Chang-Hu, ZHAO Yu-Ran, TANG Qing-Juan, XU Jia-Chao, YANG Wen-Ge. An Efficient and Rapid Method for DNA Extraction and Species Identification from Sea Cucumber Nutrient Solution[J]. FOOD SCIENCE, 2008, 29(5): 269-272 https://doi.org/

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An Efficient and Rapid Method for DNA Extraction and Species Identification from Sea Cucumber Nutrient Solution

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