Separation and Identification of Polysaccharides from Natural Boletus and Their Antixidant Activities

Abstract

Abstract: The crude polysaccharides of wild Natural Boletus were extracted by hot water and deposited by ethanol. DEAE Cellulose column chromatography was used for polysaccharides purification after the protein was removed by Seveg method,and four kinds of polysaccharides named as Nb-I,Nb-II,Nb-III and Nb-Ⅳ were separated. Infrared spectrum analysis showed that Nb-Ⅰis β-D-glucopyranose containing glucuronic acid,and Sepharose CL-4B method showed that molecular weight of NbI is 4.0×104. Both crude polysaccharides and Nb-Ⅰhave total antioxidation activity(T-AOC) ,and that of Nb-Ⅰis higher.

Key words: Natural Boletus, polysaccharide, separation and identification, total antioxidation activity

YANG Li-Hong, LIU Lin-De, ZHONG Xu-Sheng, FENG Pei-Yong, CAI De-Hua, MA Chong-Jun. Separation and Identification of Polysaccharides from Natural Boletus and Their Antixidant Activities[J]. FOOD SCIENCE, 2008, 29(8): 335-338.

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Separation and Identification of Polysaccharides from Natural Boletus and Their Antixidant Activities

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