FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (22 ): 264-267.doi: 10.7506/spkx1002-6300-200922061

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Monosaccharide Composition Analysis of Polysaccharide Gum from Crotalaria mucronata Seed

GUO Shou-jun,YANG Yong-li,ZHANG Qi-ming,SHE Jian-bao,ZHU You-chong,HONG Jun-jiang   

  1. Department of Biology, Hanshan Normal University,Chaozhou 521041, China
  • Received:2009-06-20 Online:2009-11-15 Published:2010-12-29
  • Contact: GUO Shou-jun E-mail:sjguo2006@yahoo.com.cn

Abstract:

Monosaccharides composition of the polysaccharide gum from Crotalaria mucronat seed were analyzed using thin layer chromatography (TLC), GC and HPLC methods. GC analysis indicated that there were 25.377% of D-galactose and 60.282% of D-mannose with their molar ratio at 1:2.375, while HPLC analysis indicated that there were 29.261% of D-galactose and 70.739% of D-mannose with the molar ratio at 1:2.417. The D-galactose/D-mannose molar ratio results obtained from GC and HPLC analysis were close.

Key words: Crotalaria mucronat, polysaccharide, TLC, GC, HPLC

CLC Number: