Abstract: Objective: To compare the extraction efficiencies of polysaccharides from Campanumoea javanica roots by different extraction methods. Methods: Three different extraction methods were optimized by uniform design combined with second-order polynomial regression analysis for enhanced extraction efficiency. Results: The optimal hot water extraction conditions were material-to-liquid ratio of 1:25 (g/mL), extraction temperature of 70 ℃ and extraction time of 60 min. Under these conditions, the maximum yield of polysaccharides was 28.05%. The optimal ultrasonic-assisted extraction conditions were material-to-liquid ratio of 1:50 (g/mL), ultrasonic power of 210 W and extraction time of 40 min. Under these conditions, the maximum yield of polysaccharides was 28.12%. The optimal microwave-assisted extraction conditions were material-to-liquid ratio of 1:15 (g/mL), microwave power of 900 W and extraction time of 5 min. Under these conditions, the maximum yield of polysaccharides was 28.99%. Conclusion: No significant difference was observed among three extraction methods. However, ultrasonic-assisted extraction and microwave-assisted extraction have a time-saving advantage over hot water extraction.

Key words: Campanumoea javanica, polysaccharide, hot water extraction, ultrasound-assisted extraction, microwave-assisted extraction, uniform design