Apoptosis Study on Human or Leukaemia Cell Line U937 Induced by Ginkgolic Acids from Ginkgo biloba L.

Abstract

Abstract: To investigate the inhibition effects of ginkgolic acids on U937 cells in vitro and its anti-tumor mechanism. Method: The growth inhibition effects of ginkgolic acids on U937 cell were assayed by MTT method. Changes of U937 cell morphologic character were observed under laser focusing microscope. Bio-chemistry characteristics of cell apoptosis was observed by DNA agarose electrophoresis. Apoptosis rate of U937 was analyzed by FCM. Result: Ginkgolic acids shows obvious inhibiting effect on cell growth after culturing U937 cell with it for 24～48h. Exposure U937 cell to ginkgolic acids for 28h, that ginkgolic acids make the U937 nucleolus shrink in volume, darken in color, distort in shape. U937 cells show morphologic characteristics of cell apoptosis. U937 cell DNA appeare DNA ladder type in agarose electrophoresis. Exposing U937 cell to 10.0μg/ml ginkgolic acids for 40h, the apoptosis rate is 14%. Conclusion: Ginkgolic acids show an anti-tumor activity through inducing apoptosis in tumour cell.

Key words: Ginkgolic acids, U937 cell, apoptosis, MTT(3-(4,5-Dimethylthiazol-z-yl)-2,5-Diphenyltetrazolium Bromide)

YANG Xiao-Ming, Chen-Yong-Chang, Chen-Jun, Xie-Ji-Min. Apoptosis Study on Human or Leukaemia Cell Line U937 Induced by Ginkgolic Acids from Ginkgo biloba L.[J]. FOOD SCIENCE, 2006, 27(9): 241-246.

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Apoptosis Study on Human or Leukaemia Cell Line U937 Induced by Ginkgolic Acids from Ginkgo biloba L.

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