Optimization of Multi-Enzyme Hydrolysis of Laminaria japonica for Polysaccharide Extraction

Abstract

Abstract: This study was designed to optimize the extraction of polysaccharides from Laminaria japonica by one-step hydrolysis with four different enzymes. By using orthogonal array design and analysis of variance based on one-factor-at-a-time experiments, the optimal hydrolysis conditions for polysaccharide extraction from Laminaria japonica were 0.5% cellulase, 1.0% pectinex, 1.0% papain, 1.0% xylanase, extraction temperature of 30 ℃, extraction time of 3 h and pH 5.0. Under these conditions, the extraction yield of polysaccharides was 78.9%, which was increased approximately 3-fold compared with that obtained by the traditional extraction method.

Key words: Laminaria japonica, multiple enzymes, polysaccharide

CLC Number:

TS201.1

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