Abstract:

Purpose: To investigate the effects of cyanidin-3-O-β-glucoside (Cy-3-g) on the proliferation and differentiation
of human megakaryocytes cell line (Dami). Methods: Megakaryocytes were cultured with or without different concentrations
of Cy-3-g (0.05, 0.50, 5.00, 50.00 and 100.00 mmol/L). Cell viability and proliferation were respectively measured by
typan blue staining and MTT test. Colony formation of megakaryocytes in soft agar medium from different groups was
assayed. After culturing the cells from each group, giemsa staining was used to observe cell morphology. Polyploidy of
megakaryocyte DNA and CD41 expression rate were tested by flow cytometry. Results: Cy-3-g at concentrations of 50.00
and 100.00 mmol/L effectively enhanced the viability of Dami cells when compared with the control group (P < 0.05). Dami
cells treated with Cy-3-g showed higher cell proliferation than the cells from the control group (P < 0.01). Cy-3-g at 50.00
and 100.00 mmol/L significantly increased the number of colony formation, DNA polyploidy and CD41 expression rate in
Dami cells compared to the control group (all P < 0.01). Conclusion: Cy-3-g can promote the proliferation and differentiation
of human megakaryocytes, which provides a potential theoretical basis for platelet production induced by anthocyanins.

Key words: cyanidin-3-O-β-glucoside, megakaryocyte, proliferation, differentiation