FOOD SCIENCE ›› 2019, Vol. 40 ›› Issue (15): 162-168.doi: 10.7506/spkx1002-6630-20180728-349

• Nutrition & Hygiene • Previous Articles     Next Articles

Protective Effect and Mechanism of Resveratrol on Cerebral Nerve Cells of Aging Mice

ZHANG Xianyi, SONG Yehao, LI Lu, YIN Shuhua, FU Wangwei, WU Ruiting, PAN Meng, WU Wenying, TANG Xiaofang, LI Wenjuan   

  1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
  • Online:2019-08-15 Published:2019-08-26

Abstract: Objective: To investigate the protective effect and underlying mechanism of resveratrol (RSV) on cerebral nerve cells in aging mice induced by D-galactose (D-gal). Methods: An aging mouse model was established by intraperitoneal injection of D-gal. The changes in body mass and brain index (brain/body mass ratio) were calculated. Apoptotic rate, reactive oxygen species (ROS) level and mitochondrial membrane potential (MMP) in cerebral cortical neurons were measured by flow cytometry. Mitochondrial caspase-9 and caspase-3 activity were measured, and the difference in the expression of mitochondrial and cytoplasmic cytochrome c (Cyt c) was analyzed by Western blotting. Results: There was no significant difference in body mass among all groups within the experimental period (P > 0.05). Brain index decreased significantly in the aging model group (P < 0.05), the number of times the mice crossed the hidden platform declined and the apoptotic rate of neurons significantly increased (P < 0.01) compared with the normal group, indicating that D-gal significantly damaged nerve cells in mice. Brain index in all three RSV treatment groups especially at a dose of 25.00 mg/kg mb was significantly higher than in the model group (P < 0.01), the number of times the mice crossed the hidden platform increased but with no significance (P > 0.05), and the apoptotic rate of nerve cells declined significantly (P < 0.01), leading to the conclusion that RSV has a protective effect on cerebral neural cells of D-gal-induced aging mice. ROS content in nerve cells of the model group increased significantly (P < 0.01), and MMP significantly decreased compared with the normal group (P < 0.01). Moreover, the expression of mitochondrial Cyt c decreased, the expression of cytoplasmic Cyt c increased, and mitochondrial caspase-9 and caspase-3 activity increased significantly in the model group (P < 0.01), indicating that D-gal could lead to mitochondrial damage. Compared with the model group, ROS level in nerve cells significantly decreased in the RSV groups (P < 0.01); MMP significantly increased (P < 0.01); the expression of mitochondrial Cyt c significantly increased, while the expression of cytoplasmic Cyt c significantly decreased (P < 0.05); and the activity of caspase-9 and caspase-3 significantly decreased (P < 0.01), indicating that resveratrol exerts its protective effect on nerve cells of aging mice through the mitochondrial pathways. Conclusion: Resveratrol can protect cerebral nerve cells of aging mice induced by D-gal through the mitochondrial pathways.

Key words: resveratrol, D-galactose, apoptosis, neuroprotection, mitochondrial pathway

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