In vitro pro-angiogenic property of porphyran prepared from Porphyra yezoensis in HUVECs cell model

Abstract

Abstract: Abstract: In this study, the physicochemical properties of porphyran isolated from Porphyra yezoensis including the contents of total sugar, protein, 3,6-anhydro galactose and sulfate contents, the apparent molecular weight and the monosaccharide composition were analyzed. The pro-angiogenic activity and mechanism of porphyran were further investigated using an in vitro angiogenesis model with human umbilical vein endothelial cells (HUVECs). The cell viability of HUVECs was determined by an MTT assay. Cell adhesion and migration capacities were evaluated using cell adhesion and wound healing assay. The numbers of lumens formed by HUVECs were analyzed using an in vitro tube formation assay. The expression levels of E-Cadherin and N-Cadherin in HUVECs were analyzed by western blotting, and the potential mechanism of pro-angiogenic activity of porphyran on HUVECs was further explored by MAP kinase-specific inhibitor- and VEGF receptor tyrosine kinase inhibitor-analyses. The results showed that porphyran contented total sugar of 77.48%±2.62%, protein contents of 3.24%±0.58%, 3,6-anhydro galactose contents of 20.3%±1.06%, and sulfate groups of 20.78%±3.31%. The apparent molecular weight of porphyran was 39.81 kDa. Porphyran was mainly composed of galactose, 3,6-anhydro galactose and sulfate groups, suggests that it is a sulfated galactan. Furthermore, our results showed that porphyran treatment in the range of 0~200 μg/mL has no significant toxicity to HUVECs cells. More interesting, our results revealed that porphyran can promote cell proliferation, adhesion, migration, and vascular network formation in HUVECs model. Further studies demonstrated that porphyran exerted the pro-angiogenic effect primarily via mediating down-regulation of E-Cadherin expression and up-regulation of N-Cadherin expression, through the JNK/MAPK and VEGF receptor signaling pathways. In conclusion, our findings suggest that porphyran has pro-angiogenic potential, which shed light on the precision nutrition-oriented deep processing and high-value utilization of red seaweed P.yezoensis and its polysaccharide as a novel pro-angiogenic nutrition factor.

Key words: Kedwords: Porphyran, Human umbilical vein endothelial cells, cell migration, pro-angiogenic activity

CLC Number:

TS201.4

feng fengzhen Mingjing Zheng NI Hui Ze-Dong JIANG. In vitro pro-angiogenic property of porphyran prepared from Porphyra yezoensis in HUVECs cell model[J]. FOOD SCIENCE, 0, (): 0-0.

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