食品科学 ›› 2009, Vol. 30 ›› Issue (3): 104-108.doi: 10.7506/spkx1002-6630-200903022

• 基础研究 • 上一篇    下一篇

常见药食植物天然抗氧化物质的评价与筛选

赵艳红,李建科* ,赵 维,吴晓霞   

  1. 陕西师范大学食品工程与营养科学学院
  • 收稿日期:2008-01-12 修回日期:2008-05-22 出版日期:2009-02-01 发布日期:2010-12-29
  • 通讯作者: 赵艳红 E-mail:o7812547@126.com

Evaluation on Antioxidant Activities of Extracts from Common Edible and Medicinal Plants in vitro

ZHAO Yan-hong,LI Jian-ke*,ZHAO Wei,WU Xiao-xia   

  1. (College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710062, China)
  • Received:2008-01-12 Revised:2008-05-22 Online:2009-02-01 Published:2010-12-29
  • Contact: ZHAO Yan-hong E-mail:o7812547@126.com

摘要:

本实验选用普鲁士兰法、邻二氮菲-Fe2+ 氧化法、光照核黄素- 氮蓝四唑(NBT)法和DPPH·(1,1-Diphenyl-2-Picryl-Hydrazyl)法四种常用的体外抗氧化活性评价方法,以蒸馏水、80% 乙醇和乙酸乙酯作为样品提取剂,对27 种常见的植物原料的抗氧化能力进行全面的比较分析。结果表明,不同植物体外抗氧化能力差别较大,同种植物对不同抗氧化能力也存在一定差异,而同一植物的三种提取物在同一个抗氧化评价指标上也有差距。

关键词: 药食植物, 抗氧化能力, 清除自由基

Abstract:

Four evaluation methods of antioxidants in vitro such as prussian blue spectrophotometry measure, 1,10- phenanthroline-Fe2+ oxidative assay, riboflavin photodegradation-NBT reduction method and DPPH· method were used to evaluate the antioxidant activities of distilled water, 80% ethanol and ethyl acetate extracts of 27 plant samples. The results showed that all the extracts has strong antioxidant activity. However, their antioxidant activities show significant discrepancy, having close relations with extraction solvent, plant sample cultivars and activity assay methods.

Key words: edible and medicinal plant, antioxidant activity, radical scavenging

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