食品科学 ›› 2009, Vol. 30 ›› Issue (20 ): 27-33.doi: 10.7506/spkx1002-6300-200920001

• 工艺技术 •    下一篇

蓝莓总花色苷匀浆的提取条件优化及抗氧化活性

杨 磊1,贾 佳1,2,祖元刚1,*   

  1. 1.东北林业大学 森林植物生态学教育部重点实验室 2.大庆医学高等专科学校
  • 收稿日期:2008-08-27 出版日期:2009-10-15 发布日期:2010-12-29
  • 通讯作者: 祖元刚1,* E-mail:zygorl@163.com
  • 基金资助:

    "十一五"国家科技支撑计划项目(2006BAD18B04)

Optimization of Homogenization of Blueberry Fruits for Extracting Total Anthocyanin

YANG Lei1 JIA Jia1,2 ZU Yuan-gang1,*   

  1. 1. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China
    2. Daqing Higher Medical College, Daqing 163312, China
  • Received:2008-08-27 Online:2009-10-15 Published:2010-12-29
  • Contact: ZU Yuan-gang1,* E-mail:zygorl@163.com

摘要:

以抗氧化活性为示踪,以总花色苷提取率和2,2-吖嗪双(3-乙基-7-苯并噻唑啉磺酸)二铵盐(ABTS)自由基清除能力为指标,利用匀浆法对蓝莓鲜果中的花色苷进行提取,对匀浆提取过程中的各因素在单因素试验的基础上先后采用Plackett Burman和Box-Behnken试验设计进行优化,得到蓝莓总花色苷最佳工艺条件为pH2.5、乙醇体积分数75%、料液比1:17(g/ml)、提取时间20s、提取次数1次。在此条件下,蓝莓总花色苷平均提取率为1.132%,ABTS自由基清除能力为416.8μg/ml。

关键词: 蓝莓, 总花色苷, 匀浆提取, 响应面法(RSM), 抗氧化

Abstract:

Effects of five homogenization conditions for extracting total anthocyanin from blueberry fruits such as pH of extraction solvent (acidic ethanol solution), ethanol concentration, material/liquid ratio, and homogenization time and number on total anthocyanin yield and 2,2-azinobis-(3-ethylbenzthiazoline-6-sulfonate (ABTS) radical scavenging capacity of blueberry fruits extract examined by Trolox equivalent antioxidant capacity (TEAC) assay were evaluated by single-factor method. Based on this, three main affecting factors (pH of acidic ethanol solution, ethanol concentration and material/liquid ratio) were screened by Plackett-Burman (PB) design and then optimized by Box-Behnken experimental design and response surface methodology (RSM). The optimum conditions for extracting total anthocyanin from blueberry fruits were determined as follows: using acidic 75% ethanol solution at pH 2.5 as an extraction solvent for once homogenization for 20 s at a material/liquid ratio of 1:17 (g/ml). Under these conditions, the average extraction yield of total anthocyanin was 1.132% and the capacity to scavenge ABTS radicals 416.8 μg/ml.

Key words: blueberry, total anthocyanin, homogenization extraction, response surface methodology (RSM), antioxidation

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