甘薯茎叶中异槲皮苷及咖啡酰基奎宁酸类衍生物的抗氧化活性

doi:10.7506/spkx1002-6630-201307023

食品科学 ›› 2013, Vol. 34 ›› Issue (7): 111-114.doi: 10.7506/spkx1002-6630-201307023

甘薯茎叶中异槲皮苷及咖啡酰基奎宁酸类衍生物的抗氧化活性

李佳银1,2，于欢2，石伯阳1,2，李觅路2，陆英1,2,*

1.湖南农业大学国家植物功能成分利用工程技术研究中心，湖南长沙 410128； 2.湖南农业大学园艺园林学院，湖南长沙 410128)

收稿日期:2012-01-07 修回日期:2013-02-05 出版日期:2013-04-15 发布日期:2013-03-20
通讯作者: 陆英 E-mail:canghaizhilang@126.com
基金资助:
湖南省科技厅科技计划项目(2009NK3099)；湖南省教育厅科学研究项目(10C0799；2012NK3088)

Antioxidant Activity of Isoquercitrin and Caffeoylquinic Acid Derivatives from Sweet Potato Stems and Leaves

LI Jia-yin1,2，YU Huan2，SHI Bo-yang1,2，LI Mi-lu2，LU Ying1,2,*

1. National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, Hunan Agricultural University, Changsha 410128, China；2. College of Horticulture and Gardening, Hunan Agricultural University, Changsha 410128, China

Received:2012-01-07 Revised:2013-02-05 Online:2013-04-15 Published:2013-03-20
Contact: LU Ying E-mail:canghaizhilang@126.com

摘要/Abstract

摘要： 通过测定还原能力、活性氧自由基的清除作用以及脂质过氧化抑制活性，对甘薯茎叶中含量较高的异槲皮苷(Q-Glu)和异绿原酸A，B，C(3,5-diCQA、3,4-diCQA、4,5-diCQA)4种多酚类化合物的抗氧化活性进行评估。结果显示：4种甘薯茎叶中的多酚类化合物均表现出较高的抗氧化能力，且抗氧化性与化合物浓度呈一定相关性：Q-Glu、3,5-diCQA、3,4-diCQA、4,5-diCQA对DPPH自由基的半清除浓度(IC50)分别为47.27、41.61、43.55、46.02μmol/L；对·OH的IC50值分别为190.99、151.20、151.24、217.31μmol/L；Q-Glu、3,5-diCQA、3,4-diCQA对O2－·的IC50值分别为0.81、0.46、0.71mmol/L；3,5-diCQA、3,4-diCQA、4,5-diCQA对脂质过氧化物的IC50值为3.13、2.53、4.44mmol/L，4,5-diCQA和Q-Glu在实验浓度范围内分别对O2－·清除率和对脂质过氧化物的抑制率低于50%。其中3,5-diCQA的还原能力和清除DPPH自由基、·OH、O2－·等活性氧和自由基的能力最强，3,4-diCQA对脂质过氧化反应的抑制作用最强。4种甘薯茎叶中的多酚类化合物均具有显著抗氧化能力。

关键词: 甘薯茎叶, 多酚类, 抗氧化, 活性氧自由基

Abstract: The antioxidant activity of four major polyphenolic compounds, namely quercetin-3-O-β-D-glucoside (Q-Glu), 4,5-di-O-caffeoylquinic acid (4,5-diCQA), 3,4-di-O-caffeoylquinic acid (3,4-diCQA) and 3,5-di-O-caffeoylquinic acid (3,5-diCQA) derived from sweet potato stems and leaves were evaluated based on reducing power, DPPH, hydroxyl and superoxide anion free radical scavenging, and anti-lipidperoxidation assays and compared with that of VC. These four polyphenolic compounds demonstrated strong antioxidant activity in a concentration-dependent manner. The IC50 values of Q-Glu, 3,5-diCQA, 3,4-diCQA, 4,5-diCQA against DPPH and hydroxyl free radicals were 47.27, 41.61, 43.55, 46.02 μmol/L, and 190.99, 151.20, 151.24, 217.31 μmol/L, respectively, whereas the IC50 values of Q-Glu3, 5-diCQA, and 3, 4-diCQA against superoxide anion free radical were 0.81, 0.46, 0.71 mmol/L, respectively. In anti-lipidperoxidation assay the IC50 values of 3,5-diCQA, 3,4-diCQA, and 4,5-diCQA were 4.44, 2.53, 3.13 mmol/L, respectively. Within the studied concentration range, however, 4,5-diCQA and Q-Glu did not reach 50% superoxide anion free radical scavenging and lipidperoxidation. Among these compounds, 3,5-diCQA showed the strongest reducing power and radical scavenging activity, and the antilipidperoxidation activity of 3,4-diCQA was the highest. These results show that these four polyphenolic compounds from sweet potatoes stems and leaves have significant antioxidant effects and are promising natural antioxidants.

Key words: sweet potato, polyphenols, antioxidants, reactive oxygen species

中图分类号:

R151.3

李佳银1,2，于欢2，石伯阳1,2，李觅路2，陆英1,2,*. 甘薯茎叶中异槲皮苷及咖啡酰基奎宁酸类衍生物的抗氧化活性[J]. 食品科学, 2013, 34(7): 111-114.

LI Jia-yin1,2，YU Huan2，SHI Bo-yang1,2，LI Mi-lu2，LU Ying1,2,*. Antioxidant Activity of Isoquercitrin and Caffeoylquinic Acid Derivatives from Sweet Potato Stems and Leaves[J]. FOOD SCIENCE, 2013, 34(7): 111-114.

参考文献

[1] WINTERBOURN C C. Reconciling the chemistry and biology of reactive oxygen species [J]. Nature Chemical Biology, 2008, 4, 278-286.
[2] APEL K, HIRT H. Reactive oxygen species: metabolism, oxidative stress, and signal transduction [J]. Annual Review of Plant Biology, 2004, 55(0): 373-399.
[3] ISLAM S. Sweetpotato (ipomoea batatas l.) leaf: Its potential effect on human health and nutrition [J]. Agric. Food, 2006, 71(2): 13-121.
[4] KANO M, TAKAYANAGI T, HARADA K, et al. Antioxidative activity of anthocyanins from purple sweet potato, Ipomoera batatas cultivar Ayamurasaki [J]. Bioscience, Biotechnology, and Biochemistry, 2005, 69(5): 979-988.
[5] KURATA R, ADACHI M, YAMAKAWA O, et al. Growth suppression of human cancer cells by polyphenolics from sweetpotato (ipomoea batatas l.) leaves [J]. Agric. Food Chem, 2007, 55: 185-190.
[6] POCHAPSKI M T, FOSQUIERA E C, ESMERINO L A, et al. Phytochemical screening, antioxidant, and antimicrobial activities of the crude leaves' extract from ipomoea batatas (L.)Lam [J]. Pharmacognosy Magazine, 2011, 7(26): 165-170.
[7] ROBINSON W E, CORDEIRO M, ABDEL-MALEK S, et al. Dicaffeoylquinic acid inhibitors of human immunodeficiency virus integrase: Inhibition of the core catalytic domain of human immunodeficiency virus integrase [J]. Mol. Pharmacol, 1996, 50(4): 846-855
[8] ROYHAN A, SUSILOWATI RINA, SUNARTI. Effects of white-skinned sweet potato (ipomoea batatas l.) on pancreatic beta cells and insulin expression in streptozotocin induced diabetic rats [J]. Majalah Kesehatan PharmaMedika, 2011, 1(2): 45-49.
[9] TRUONG V D, MCFEETERS RF, THOMPSON RT, et al. Phenolic acid content and composition in leaves and roots of common commercial sweetpotato (ipomea batatas L.) cultivars in the United States [J]. Food Sci., 2007, 72(6): C343-349.
[10] ZHU Fan, CAI Yizhong, YANG Xinsun, et al. Anthocyanins, hydroxycinnamic acid derivatives, and antioxidant activity in roots of different chinese purple-fleshed sweetpotato genotypes[J]. Agric. Food Chem., 2010, 58(13): 7588-7596.
[11] JUNG J K, LEE S U, KOZUKUE N, et al. Distribution of phenolic compounds and antioxidative activities in parts of sweet potato (ipomoea batata L.) plants and in home processed roots[J]. Agric. Food Chem., 2010, 24(1): 29-37.
[12] TAN G S, XU P S, DAI Z Y, et al. Studies on the chiemical compounds of ipomoea batatas Lam [J]. Nat. Prod. Res. Develop., 1995, 9(4): 44-46.
[13] AMAROWICZ R, ZEGARSKA Z, RAFALOWSKI R, et a1. Antioxidant activity and free radicalscavenging capacity of ethanolic extracts of thyme, oregano, and marjoram [J]. Eur J Lipid Sci Technol., 2009, 111(11): 1111-1117.
[14] OYAIZU M．Studies on products of browning reaction: antioxidative activies of products of browning reaction prepared from glucosamine [J]. Japanese J Nutr., 1986, 44(6): 307-315．
[15] 马庆一, 张侠, 熊卫东, 等. 红薯梗中清除自由基活性成分的研究[J]. 食品科学, 2003, 24(6): 145-148.
[16] 陆志科, 黎深. 荔枝核活性成分分析及其提取物抗氧化性能研究[J]. 食品科学, 2009, 30(23): 110-113.
[17] 张宇思, 龚祝南, 石雪萍. 花椒总黄酮的抗氧化作用[J]. 食品科学, 2011, 32(15): 70-73.
[18] 王莹. 茶多酚的抗氧化和抑菌活性及其增效剂[J]. 生物学杂志, 2007, 24(5): 54-56.
[19] DANDONA P, ALJADA A. Bandyopadhyay A. Inflammation: the link between insulin resistance, obesity and diabetes [J]. Trends Immunol. 2004, 25(1):4-7

甘薯茎叶中异槲皮苷及咖啡酰基奎宁酸类衍生物的抗氧化活性

Antioxidant Activity of Isoquercitrin and Caffeoylquinic Acid Derivatives from Sweet Potato Stems and Leaves

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