食品科学

• 基础研究 • 上一篇    下一篇

不同品种苹果籽总酚含量与抗氧化相关性研究

徐 颖1,2,樊明涛1,*,冉军舰3,程拯艮1,戚一曼1   

  1. 1.西北农林科技大学食品科学与工程学院,陕西 杨凌 712100;
    2.陕西科技大学生命科学与工程学院,陕西 西安 710021;3.河南科技学院食品学院,河南 新乡 453003
  • 出版日期:2015-01-15 发布日期:2015-01-16

Correlation between Total Phenolic Content and Antioxidant Activity in Apple Seeds from Seven Cultivars

XU Ying1,2, FAN Mingtao1,*, RAN Junjian3, CHENG Zhenggen1, QI Yiman1   

  1. 1. College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China;
    2. College of Life Science and Engineering, Shaanxi University of Science and Technology, Xi’an 710021, China;
    3. School of Food Science, Henan Institute of Science and Technology, Xinxiang 453003, China
  • Online:2015-01-15 Published:2015-01-16

摘要:

为探讨苹果籽总酚含量与抗氧化相关性及其抗氧化的物质基础,以7 个品种苹果籽为材料,采用 Folin-Ciocalteu法测定总酚含量,FRAP法、ABTS+·法、O2-·清除法、·OH清除法和DPPH自由基清除法评价抗氧化活性,高效液相色谱法进行多酚成分分析。结果表明:7 个品种苹果籽总酚含量范围为5.74~17.44 mg GAE/g(以干质量计),抗氧化性优于茶多酚、VC和2,6-二叔丁基-4-甲基苯酚(2,6-di-tert-butyl-4-methylphenol,BHT),蜜脆苹果籽具有最高的总酚含量和最好的抗氧化能力。除DPPH自由基清除法外,多酚含量与其他4 种方法测得的抗氧化数据具有显著正相关(r=0.771~0.984)。根皮苷为苹果籽多酚抗氧化的主要成分。

关键词: 苹果籽, 总酚, 抗氧化, 相关性

Abstract:

This study aimed to investigate the correlation between the total phenolic content and antioxidant activity in apple
seeds from seven cultivars. The total phenolic content was determined by Folin-Ciocalteu method, the antioxidant activity
was evaluated by total reducing capacity, 3-ehtylbenzothiazolin-6-sulfnic acid diammonium salt (ABTS) scavenging,
superoxide anion scavenging, hydroxyl free radical scavenging and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical
scavenging assays, and the polyphenol composition of apple seeds was analyzed by high performance liquid chromatography
(HPLC). It was shown that Honeycrisp exhibited the highest total phenolic content and the best antioxidant properties among
seven apple cultivars. Antioxidant activity of polyphenols in apple seeds was significantly positively correlated with total
phenolic content (r = 0.771–0.984), with the exception of DPPH scavenging capacity. Compared to tea polyphenols, vitamin
C (VC) and 2,6-di-tert-butyl-4-methylphenol (BHT), apple seed polyphenols exhibited better antioxidant activity. Phloridzin,
which accounted for 79.76%-86.67% of the total phenolic content as determined by HPLC, was the dominant antioxidant
component in apple seeds. Polyphenols extracted from apple seeds could have a wide application prospect because they act
as the safe, non-toxic natural antioxidants.

Key words: apple seeds, total phenolic, antioxidant, correlation

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