关键词: 赭曲霉毒素A, 免疫亲和层析净化, 高效液相色谱

Abstract: A rapid and accurate method for the determination of ochratoxin A(OTA) in foods was established. Samples were extracted by methanol-water or NaHCO3 solution. After dilution the extarcts,flowed through a OchraTest column containing specific antibody of OTA. The column was eluted with PBS buffer,OTA buffer,pure water,and methanol in turn. The methanol eluate was separated on C18(150×4.60 mm,5μm) at 35 ℃ with acetonitrile-water-acetic acid(99:99:2,V/V) as mobile phase at the elution flow rate 0.90 ml/min. The excitation wavelength of fluorescence detector was 333 nm and the emission wavelength was 477 nm. It was found that there is a good linear relationship between the peak area and the concentration. The recoveries of OTA are 90.3% to 106%,and the relative standard deviations are1.75% to 4.32% for different kinds of food sample. The method is simple,rapid,accurate and applicable for the determination of OTA in foods.

Key words: ochratoxin A, immunoaffinity clean-up, HPLC