食品科学

• 基础研究 • 上一篇    下一篇

南酸枣在模拟消化过程中抗氧化活性及多酚含量分析

王谢祎1,翟宇鑫1,李 倩1,戴涛涛1,陈 军1,李 俶1,*,刘继延2,刘成梅1   

  1. 1.南昌大学 食品科学与技术国家重点实验室,江西 南昌 330047;2.江西齐云山食品有限公司,江西 赣州 341000
  • 出版日期:2016-06-15 发布日期:2016-06-27

Changes in Antioxidant Activity and Phenol Content in Choerospondias axillaris Fruits during Simulated Gastrointestinal Digestion

WANG Xieyi1, ZHAI Yuxin1, LI Qian1, DAI Taotao1, CHEN Jun1, LI Ti1,*, LIU Jiyan2, LIU Chengmei1   

  1. 1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China;
    2. Jiangxi Qiyunshan Food Co. Ltd., Ganzhou 341000, China
  • Online:2016-06-15 Published:2016-06-27

摘要:

研究南酸枣在体外模拟胃肠消化过程中酚类化合物的含量变化(没食子酸、儿茶素、表儿茶素)及其抗氧化性的变化。采用高效液相色谱法测定了3 种酚类物质的含量;同时用总抗氧化能力法、2,2’-连氮基-双-(3-乙基苯并二氢噻唑啉-6-磺酸)(2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate),ABTS)自由基清除法和羟自由基(·OH)清除法分别测定南酸枣消化过程中的抗氧化性。结果表明:胃肠液模拟消化后3 种酚类物质含量下降,但均无显著变化。南酸枣经体外模拟消化后,其抗氧化能力逐渐下降。本实验对南酸枣消化过程中的多酚组成和抗氧化性进行研究,发现随着多酚含量的减少,抗氧化性逐渐降低。并通过比较胃肠消化过程的两个因素(化学因素、酶活性因素)的影响,发现消化酶对酚类物质含量的影响较小,而主要是化学环境影响了酚类物质含量,但是消化酶可显著提高南酸枣在消化过程中的抗氧化性。

关键词: 南酸枣, 模拟胃肠消化, 抗氧化作用, 高效液相色谱

Abstract:

The purpose of this work was to explore the phenol composition and antioxidant activity during in vitro simulated
gastrointestinal digestion of Choerospondias axillaris fruits. The contents of galic acid, catechin and epicatechin in
Choerospondias axillaris fruits were determined using high performance liquid chromatography (HPLC). Meanwhile, the
antioxidant activity was evaluated by measuring total antioxidant capacity, ABTS (2,2’-azinobis-(3-ethylbenzthiazoline-
6-sulphonate) radical and hydroxyl radical scavenging capacity. The results showed that the contents of three phenol
components decreased in spite of no statistical significance after the simulated digestion. Similarly, the antioxidant capacity
declined. Further this study found that the antioxidant activity decreased with the reduction in the phenolic contents. In
comparison with chemical factors, digestive enzymes had less impact on the of phenolic contents, whereas changes in
the chemical environment resulted in a significant change in the phenolic contents. Digestive enzymes could significantly
improve the antioxidant activity during gastrointestinal digestion.

Key words: Choerospondias axillaris fruit, in vitro simulated gastrointestinal digestion, antioxidant activity, high performance liquid chromatography (HPLC)

中图分类号: