• 成分分析 •

HPLC测定番荔枝果实中酚类成分及抗氧化相关性

1. 南京中医药大学药学院，江苏 南京 210023
• 出版日期:2019-06-25 发布日期:2019-06-28
• 基金资助:
国家自然科学基金面上项目（81573577；81403082；81274057）

HPLC Determination of Phenolic Compounds in Three Fruit Parts of Annona squamosa Linn. and Their Antioxidant Activities

HAN Rui, CHEN Yayun, JI Junyang, CHEN Yong, LI Xiang*, CHEN Jianwei

1. College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China
• Online:2019-06-25 Published:2019-06-28

Abstract: Objective: To establish a high performance liquid chromatographic (HPLC) method for the simultaneous determination of the contents of 13 phenolic constituents in three fruit parts of Annona squamosa Linn., and to analyze the correlation between phenol content and antioxidant activity. Methods: Total phenols were evaluated by a colorimetric method, and the HPLC method was used to measure the composition and contents of phenols. The antioxidant activity was analyzed using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation scavenging activity assays. Results: The total phenol content of peel was the highest, ranging from 59.48 to127.84 mg/g, sequentially followed by pulp and seeds. The contents of some individual phenolic compounds in peel significantly higher than in any other parts. There were significant regional differences in the composition and contents of phenols in the same fruit parts. The contents of p-hydroxybenzoic acid, catechin, caffeic acid and (-)-epicatechin were relatively higher in peel and pulp and the highest (-)-epicatechin level was 2 398.17 mg/mL. All of the fruit parts were had more potent scavenging activity against DPPH radical than against ABTS radical cation and peel had the strongest radical scavenging with the highest percentage inhibition of DPPH radical and ABTS radical cation being observed at concentrations of 0.39 and 1.56 mg/mL, respectively. Conclusion: The composition and contents of phenols in the peel, pulp and seeds of A. squamosa fruit are significantly different. The peel contains the highest number and amount of phenols and possesses the highest antioxidant activity, indicating that it can be considered as a potential and valuable source of natural antioxidants.