食品科学 ›› 2009, Vol. 30 ›› Issue (18 ): 34-38.doi: 10.7506/spkx1002-6300-200918003

• 工艺技术 • 上一篇    下一篇

乙醇提取莲子抗氧化物质的最佳条件研究

王西西,郑铁松* ,李起弘   

  1. 南京师范大学金陵女子学院食品科学与营养系
  • 收稿日期:2009-06-08 出版日期:2009-09-15 发布日期:2010-12-29
  • 通讯作者: 郑铁松 E-mail:tieszheng@sina.com
  • 基金资助:

    江苏省高校自然科学基金项目(07KJD550115)

Optimization of Ethanol Extraction Conditions of Antioxidant Compounds from Lotus Seeds Using Response Surface Methodology

WANG Xi-xi,ZHENG Tie-song*,LI Qi-hong   

  1. Department of Food Science and Nutrition, Ginling College, Nanjing Normal University, Nanjing 210097, China
  • Received:2009-06-08 Online:2009-09-15 Published:2010-12-29
  • Contact: ZHENG Tie-song E-mail:tieszheng@sina.com

摘要:

用清除1,1- 二苯基-2- 苦基苯肼(DPPH)自由基能力表示莲子乙醇提取物的抗氧化能力,在单因素试验基础上,选择提取时间、乙醇体积分数、提取温度为自变量,DPPH 自由基清除率为响应值,利用Box-Benhnken 中心组合试验和响应面分析法,研究各自变量及其交互作用对DPPH 自由基清除率的影响,模拟得到二次多项式回归方程的预测模型,并确定最佳提取条件为: 提取时间6.0h,乙醇体积分数100%,提取温度78℃,在此条件下,DPPH 的清除率为76.17%。

关键词: 莲子, 抗氧化, DPPH 自由基, 响应面分析法

Abstract:

Compounds having DPPH radical scavenging activity were extracted from lotus seeds using ethanol. On the basis of single-factor experiments, the effects of extraction time, ethanol concentration and temperature and interactions among them on DPPH radical scavenging activity of ethanol extract from lotus seeds using a three-factor, three-level Box-Behnken experimental design combining with response surface modeling (RSM) and a quadratic polynomial regression model describing the relationship of DPPH radical scavenging activity with the three variables was established. The optimum extraction conditions were determined as follows: ethanol concentration 100% and temperature 78 ℃ for an extraction duration of 6.0 h. Under the optimized conditions, the actual value of DPPH free radical scavenging activity was 76.17%, close to the predicted.

Key words: lotus-seeds, antioxidation, DPPH free radicals, response surface methodology

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