婴幼儿配方食品中叶黄素的提取及测定

doi:10.7506/spkx1002-6630-201204039

食品科学 ›› 2012, Vol. 33 ›› Issue (4): 189-192.doi: 10.7506/spkx1002-6630-201204039

婴幼儿配方食品中叶黄素的提取及测定

单艺，姜毓君，陈一，王象欣，鄂来明

1.东北农业大学黑龙江省乳品工业技术开发中心 2.河北北方学院农林科技学院

出版日期:2012-02-25 发布日期:2012-02-14

Extraction and Determination of Lutein in Formulated Foods for Infant and Young Children

SHAN Yi，JIANG Yu-jun，CHEN Yi，WANG Xiang-xin，E Lai-ming

1. Heilongjiang Dairy Industry Technical Development Center, Northeast Agricultural University, Harbin 150086, China； 2. College of Agricultural and Forestry, Hebei North University, Zhangjiakou 075000, China

Online:2012-02-25 Published:2012-02-14

摘要/Abstract

摘要： 比较婴幼儿配方食品中叶黄素的3种提取方法：氢氧化钾皂化法、丙酮提取法和正己烷提取法，从中得出最佳提取方法为正己烷萃取法。选用色谱柱YMC Carotenoid(4.6mm×250mm，5μm)以甲醇、甲基叔丁基醚为流动相进行梯度洗脱，流速为1mL/min，于445nm波长处测定婴幼儿配方食品中叶黄素的含量。该方法操作简便，提取效率高(平均回收率为95.4%)，重现性好(RSD为0.93%)，检出限为2μg/100g，可以准确的测定婴幼儿配方食品中叶黄素的含量。

关键词: 叶黄素, 婴幼儿配方食品, 高效液相色谱, 提取

Abstract: Three methods for extracting lutein from formulated foods for infant and young children such as potassium hydroxide saponification, acetone extraction and n-hexane extraction were compared, and the n-hexane extraction method was found to be the best of them. An HPLC method for the determination of lutein was developed based on the selected extraction method. The chromatographic separation was performed on a YMC Carotenoid (4.6 mm × 250 mm, 5μm) using a mobile phased composed of methanol and methyl tertiary butyl ether under a gradient elution mode at a flow rate of 1 mL/min. Lutein was detected at the wavelength of 445 nm. The method was characterized by easy operation and high extraction efficiency (95.4% average recovery), and good reproducibility (0.93% RSD). The detection limit was 2μg/100 g. It allowed accurate determination of lutein in formulated foods for infant and young children.

Key words: lutein, formulated foods for infant and young children, high performance liquid chromatography, extraction

中图分类号:

TS252.7

单艺，姜毓君，陈一，王象欣，鄂来明. 婴幼儿配方食品中叶黄素的提取及测定[J]. 食品科学, 2012, 33(4): 189-192.

SHAN Yi，JIANG Yu-jun，CHEN Yi，WANG Xiang-xin，E Lai-ming. Extraction and Determination of Lutein in Formulated Foods for Infant and Young Children[J]. FOOD SCIENCE, 2012, 33(4): 189-192.

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婴幼儿配方食品中叶黄素的提取及测定

Extraction and Determination of Lutein in Formulated Foods for Infant and Young Children

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