食品科学 ›› 2011, Vol. 32 ›› Issue (8): 238-243.doi: 10.7506/spkx1002-6630-201108053

• 分析检测 • 上一篇    下一篇

固相萃取-高效液相色谱检测葡萄酒中罗丹明B

李小燕1,2,李 梅1,陈其锋1,韦升艳1,罗 予1,仝海娟1   

  1. 1.广西民族大学化学与生态工程学院 2.广西林产化学品开发与应用重点实验室
  • 出版日期:2011-04-25 发布日期:2011-04-12
  • 基金资助:
    广西自然科学基金项目(2010GXNSFA013047);广西教育厅科研项目(200911LX84)

Determination of Rhodamine B in Red Wine by Solid Phase Extraction-High Performance Liquid Chromatography

LI Xiao-yan1,2,LI Mei1,CHEN Qi-feng1,WEI Sheng-yan1,LUO Yu1,TONG Hai-juan1   

  1. 1. College of Chemistry and Ecological Engineering, Guangxi University for Nationalities, Nanning 530006, China; 2. Key Laboratory of Development and Application of Forest Chemicals of Guangxi Province, Nanning 530006, China
  • Online:2011-04-25 Published:2011-04-12

摘要: 建立固相萃取富集净化,高效液相色谱紫外检测葡萄酒中禁用色素罗丹明B的方法。葡萄酒样品中的罗丹明B经C18固相萃取,40%乙醇溶液清洗净化,80%的甲醇溶液洗脱后,用高效液相色谱紫外检测器进行测定。结果表明:罗丹明B在1.0~10.0μg/mL范围呈现良好的线性关系,相关系数0.9924;在1.5~3.5μg/mL添加水平时,平均回收率为72.06%,相对标准偏差为2.87%(n=5);定量测出限为0.25μg/mL。表明该方法灵敏度高,重复性良好,操作简便快速,适用于葡萄酒中罗丹明B的检测。

关键词: 固相萃取, 罗丹明B, 葡萄酒, 高效液相色谱

Abstract: A solid phase extraction followed by high performance liquid chromatographic method was developed for the analysis of the prohibited colorant rhodamine B in red wine. The separation of rhodamine B from wines was achieved on a Supelclean- C18 SPE column washed with 40% ethanol aqueous solution (V/V) and eluted with 80% methanol aqueous solution (V/V) prior to analysis on a high performance liquid chromatography equipped with a UV detector. The calibration curve of the method was linear over the range of 1.0 to 10.0μg/mL with a correlation coefficient of 0.9924. The recoveries for rhodamine B standard spiked in the range of 1.5 to 3.5μg/mL were between 69.67% and 75.00% with a relative standard deviation (RSD) of 2.87% (n = 5). The detection limit was 0.25μg/mL. Owing to advantages of good linearity, low limit of detection, high precision and rapidity, this method is suitable for the determination of rhodamine B in red wine.

Key words: solid phase extraction, high performance liquid chromatography, rhodamine B, red wine

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