Isolation, Identification and Anti-oxidant Activity of Polysaccharides from Pholiota adiposa Mycelium

doi:10.7506/spkx1002-6300-200923028

Abstract

Abstract:

Crude polysaccharides were obtained from Pholiota adiposa Mycelium through hot-water extraction and ethanol precipitation. Crude polysaccharides after removing protein through Sevage method were purified using DEAE-cellulose column chromatography to obtain two polysaccharide ingredients, designated as PAMP-I and PAMP-II. Glucuronic acid β-D-pyranglucan was identified in both purified polysacchride components through IR spectrum analysis. Total anti-oxidant capability, scavenging capability to superoxide anion and hydroxyl free radicals, and effect of polysaccharides from Pholiota adiposa Mycelium on MDA content were evaluated using colorimetry. Results indicated that polysaccharides from Pholiota adiposa Mycelium had anti-oxidant capability such as scavenging free radicals and suppressing MDA generation in a dose-dependent mode.

Key words: Pholiota adiposa Mycelium, polysaccharide, anti-oxidant capability

CLC Number:

YANG Li-hong，HUANG Qing-rong，FENG Pei-yong，CAI De-hua，DI Hui-ling. Isolation, Identification and Anti-oxidant Activity of Polysaccharides from Pholiota adiposa Mycelium[J]. FOOD SCIENCE, 2009, 30(23 ): 131-134.

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Isolation, Identification and Anti-oxidant Activity of Polysaccharides from Pholiota adiposa Mycelium

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