Effect of Ethanol Extract of Propolis on Proliferation and Apoptosis of Human Liver Carinoma Hep G2 Cells

doi:10.7506/spkx1002-6630-201021078

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (21): 344-348.doi: 10.7506/spkx1002-6630-201021078

• Nutrition & Hygiene • Previous Articles Next Articles

Effect of Ethanol Extract of Propolis on Proliferation and Apoptosis of Human Liver Carinoma Hep G2 Cells

WU Zheng-shuang1,2，DONG Jie2，ZHANG Hong-cheng2，GAO Wen-hong1,*

1. College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641, China ；
2. Bee Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100093, China

Received:2010-05-10 Online:2010-11-15 Published:2010-12-29
Contact: GAO Wen-hong E-mail:zzhc@sohu.com

Abstract

Abstract:

In order to investigate the effect of propolis on cell proliferation and apoptosis, the ethanol extract of propolis was used to treat human liver carcinoma Hep G2 cells. The inhibitory rate of ethanol extract from propolis on the proliferation of human liver carcinoma Hep G2 cells was determined by MTT assay and the apoptosis of human liver carcinoma Hep G2 cells was examined by using inverted fluorescence microscope and flow cytometry analysis. Results indicated that 12.5－200 μg/mL ethanol extract of propolis exhibited an obvious inhibition effect on the proliferation of Hep G2 cells after 24 h and 48 h treatment in a time- and dose-dependent manner. The apoptosis of Hep G2 cells was obviously observed after treatment and apoptosis rate was in the range of 6.36% to 21.9% in a dose-dependent manner. These results confirm that propolis can significantly inhibit the proliferation and induce apoptosis of Hep G2 cells.

Key words: propolis, Hep G2 cell, proliferation, apoptosis

CLC Number:

S896.6

WU Zheng-shuang1,2，DONG Jie2，ZHANG Hong-cheng2，GAO Wen-hong1,*. Effect of Ethanol Extract of Propolis on Proliferation and Apoptosis of Human Liver Carinoma Hep G2 Cells[J]. FOOD SCIENCE, 2010, 31(21): 344-348 https://doi.org/10.7506/spkx1002-6630-201021078

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Effect of Ethanol Extract of Propolis on Proliferation and Apoptosis of Human Liver Carinoma Hep G2 Cells

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