Protective Effect of Grape Seed Proanthocyanidin Extract against Cisplatin-Induced Apoptosis in Human Embryonic Kidney Cells

doi:10.7506/spkx1002-6630-201401046

Abstract

Abstract:

Purpose: To explore the protective effect of grape seed proanthocyanidin extract (GSPE) against cisplatin
(CDDP)-induced apoptosis in human embryo kidney (HEK) 293 cells and its possible mechanisms. Methods: The effects of
CDDP and GSPE on the in vitro growth of HEK 293 cells and the effect of GSPE on CDDP-induced toxicity was evaluated
by MTT assay. CDDP-induced apoptosis in HEK293 cells was analyzed by flow cytometry. Western blotting was applied
to determine the expression of apoptotic gene Bax and anti-apoptosis gene Bcl-2. Results: GSPE was able to antagonize
cisplatin-induced cytotoxicity in a concentration-dependent manner. Flow cytometry revealed that GSPE could lead to a
decrease in apoptosis rate and pro-apoptotic Bax gene expression while anti-apoptotic gene Bcl-2 expression was enhanced.
Conclusion: GSPE demonstrates a preventive effect on cisplatin-induced injury in HEK293 cells. The mechanism may be
related to its capacity of enhancing anti-apoptotic gene Bcl-2 expression and inhibiting pro-apoptotic gene Bax expression.

Key words: grape seed proanthocyanidin extract, cisplatin, human embryo kidney (HEK) 293 cells, apoptosis

CLC Number:

Q946.8

GUO Zhuo-yu, GAO Li-ping*, LI Zhen. Protective Effect of Grape Seed Proanthocyanidin Extract against Cisplatin-Induced Apoptosis in Human Embryonic Kidney Cells[J]. FOOD SCIENCE, 2014, 35(1): 234-238.

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