Extraction, Purification and Identification of Polysaccharide from the Gonad of the Sea Urchin Strongylocentrotus intermedius

doi:10.7506/spkx1002-6630-201421004

Abstract

Abstract:

A water-soluble polysaccharide (SUGP) from the gonad of the sea urchin Strongylocentrotus intermedius was
obtained and its structural characteristics were elucidated. SUGP was isolated by protease-assisted aqueous extraction,
precipitated with ethanol, deproteinized by the Sevag method, further treated with alpha-amylase for the removal of
glycogen, and finally purified with gel filtration chromatography. The structural characteristics of SUGP were determined
by IR spectroscopy, ultraviolet spectroscopy and high performance liquid chromatography. The results showed that SUGP
was a homogeneous polysaccharide with average molecular weight of 4 436 D and contained only mannose. Alpha-amylase
eliminated the glycogen present in SUGP, so that producing a purified polysaccharide composed of only mannose, indicating
that the sea urchin polysaccharide is a glucomannan that is constructed with polymanna conjugated to glycogen.

Key words: sea urchin gonad, polysaccharide, polymanna, glycogen

CAO Yue-gang, ZHAO Jun, CHEN Hong-yu, CHEN Ning, TIAN Heng, HE Dong-mei, YANG Jing-feng. Extraction, Purification and Identification of Polysaccharide from the Gonad of the Sea Urchin Strongylocentrotus intermedius[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201421004.

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Extraction, Purification and Identification of Polysaccharide from the Gonad of the Sea Urchin Strongylocentrotus intermedius

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