Abstract:

The antitumor efficacy and mechanism of the polypeptide mixture from bay scallop were studied by CCK-8 assays
using inverted microscope and fluorescence-activated cell sorter (FACS). The results showed that different concentrations
of the polypeptide mixture from bay scallop (4, 6, 8, 10 and 12 mg/mL) could suppress the growth of human hepatocellular
carcinoma cells HepG2, and the growth inhibitory rate increased with increasing concentration of the polypeptide mixture.
After treated with the polypeptide mixture at a dose of 12 mg/mL, the morphology of HepG2 cells was changed, showing
cell shrinkage, decreased transparency and nuclear chromatin pyknosis as well as the appearance of apoptotic bodies. To
conclude, the polypeptide mixture from bay scallop can induce apoptosis of HepG2 cells.

Key words: polypeptide from bay scallop, HepG2 cell, apoptosis