In Vitro Assay on Anti-proliferation Effects of Soybean Isoflavones on Rat Breast Carcinoma Cell

Abstract

Abstract: To study the mechanism of growth inhibition of rat breast carcinoma cells by soybean isoflavones, cancer cell line was treated invitro with daidzein and genistein. Microculture tetrazolium(MTT)assay, 3H-TdR incorporation, and cell cycle flow cytometry were usedto study the inhibitory effects of daidzein and genistein on cell proliferation. Results showed that daidzein and genistein could result in adose-dependent and time-dependent growth inhibition and decrease 3H-TdR incorporation in DNA of rat breast carcinoma cell. Flowcytometry showed that cells were accumulating in the S phase, but cell apoptosis was not induced.

Key words: soybean isoflavones, breast carcinoma cell, DNA synthesis, cell cycle, apoptosis

LIN Cheng-Zhao, ZOU Si-Xiang, CHEN Wei-Hua. In Vitro Assay on Anti-proliferation Effects of Soybean Isoflavones on Rat Breast Carcinoma Cell[J]. FOOD SCIENCE, 2004, 25(7): 168-171.

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In Vitro Assay on Anti-proliferation Effects of Soybean Isoflavones on Rat Breast Carcinoma Cell

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