Effect of Kudzu Flavonoids Extracts on Expression of bcl-2 and Bax Gene in HL-60 Cell

Abstract

Abstract: Objective: To study the effect of kudzu flavonoids extracts on the expression of bcl-2 and bax gene in HL-60 cells.Methods: To use RT-PCR and western blotting technology to detect the mRNA and protein expression level of bcl-2 and bax gene in the HL-60 cells treated by kudzu flavonoids extracts, puerarin and daidzein. Results: In a time-dependent manner, kudzu flavonoids extracts, puerarin and daidzein increased the bax mRNA and protein expression level and lowered the bcl-2 protein expression level. Conclusion: The ability of kudzu flavonoids extracts could induce HL-60 cells apoptosis in relation to lower the bcl-2 mRNA and protein expression level and increase the bax mRNA and protein expression level.

Key words: kudzu flavonoids, bcl-2, bax, HL-60 cells, apoptosis

YUAN Huai-Bo, LING Qing-Zhi, MI Man-Tian, CHEN Zong-Dao. Effect of Kudzu Flavonoids Extracts on Expression of bcl-2 and Bax Gene in HL-60 Cell[J]. FOOD SCIENCE, 2005, 26(11): 241-243.

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Effect of Kudzu Flavonoids Extracts on Expression of bcl-2 and Bax Gene in HL-60 Cell

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