Study on Content Detemination and Preliminary Analysis by Thin Layer Chromatography of Total Flavonids in Different Parts of Cancrinia discoidea (Ledeb.) Poljak

Abstract

Abstract: Extraction of the total flavonids in different parts of Cancrinia discoidea (Ledeb.) Poljak by refluxing with 70% ethanol was studied.The total flavonids were determined by spectrophotometry,rutin as control sample.The results showed that the content of total flavonids in leaf was the highest, in flower was less than in leaf,and in stem was the least.We also executed qualitative analysis and comparison on the total flavonids in different parts of Cancrinia discoidea (Ledeb.) Poljak by thin layer chromatography. N-butyl alcohol-acetic acid-distilled water(4:1:5)was used as the developing solvent. The results showed that there were not only the same flavonids constituents but also different constituents in different parts of Cancrinia discoidea (Ledeb.) Poljak.

Key words: Cancrinia discoidea (Ledeb.) Poljak, different parts, total flavonids, spectrophotometry, thin layer chromatography

WU Chun-Xia, SONG Man-Shu, A Bu-Du-La-·A-Ba-Si. Study on Content Detemination and Preliminary Analysis by Thin Layer Chromatography of Total Flavonids in Different Parts of Cancrinia discoidea (Ledeb.) Poljak[J]. FOOD SCIENCE, 2007, 28(7): 430-433.

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Study on Content Detemination and Preliminary Analysis by Thin Layer Chromatography of Total Flavonids in Different Parts of Cancrinia discoidea (Ledeb.) Poljak

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