Study on Inhibition Effects of Phycoerythrin from Gracilaria lemaneiformis on Hela Cells and Its Mechanism

Abstract

Abstract: Objective:To investigate the inhibition effects of phycoerythrin(PE)from Gracilaria lemaneiftheormis on growth of Hela cells and its mechanism.Method:The proliferation of cell lines of Hela in vitro was assayed by MTT method.Effects of PE on Hela cells cycle and apoptosis were observed by flow cytometry and Annexin V-FITC/PI fluorescence staining.Result:PE could obviously inhibit the proliferation of Hela cells with a fair dose-effect relationship(p<0.01,r =0.84).The inhibitory rate at 48 h could reach more than 70% and its half inhibitory concentration(IC50)was 4.12μg/ml.PE could prevent the transformation of Hela cell cycle from G2/M to S phase to cause apoptosis.Conclusion:PE shows potent inhibitory effects on growth of Hela cells,and the mechanism is related to inducing the apoptosis of Hela cells.

Key words: phycoerythrin from Gracilaria lemaneiformis, Hela cell, apoptosis

CHEN Mei-Zhen, GE An-Shan, CUI Peng-Ju, LIAO Zao-Hui. Study on Inhibition Effects of Phycoerythrin from Gracilaria lemaneiformis on Hela Cells and Its Mechanism[J]. FOOD SCIENCE, 2007, 28(9): 549-552.

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Study on Inhibition Effects of Phycoerythrin from Gracilaria lemaneiformis on Hela Cells and Its Mechanism

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