Abstract:

Single factor and orthogonal array design methods were used to investigate an optimal procedure for the extraction of cordycepin from cultured mycelia of Cordyceps militaris with the assistance of microwave combined with ultrasonic. Besides, a UV spectroscopic method was developed for the assay of cordycepin and its figures of merit were evaluated. Results showed that the optimal values of crucial technological parameters affecting cordycepin yield were as follows: ethanol concentration 70%, microwave power 200 W, material/liquid ratio 1:240 (g/mL) and extraction time 110 s. Under such conditions, a cordycepin yield of up to 9.228 mg/g was obtained. The developed analytical method exhibited mean recoveries ranging from 97.41% to 99.60% of 3 replicates for cordycepin in a real sample spiked at 3 levels and both high accuracy and precision so as to provide a convenient and cost saving method for the assay of cordycepin.

Key words: Cordyceps militaris, cordycepin, microwave-ultrasonic assisted extraction, UV spectrophotometry