Induction and Mechanism of HeLa Cell Apoptosis by Bioactive Peptide from Oyster

Abstract

Abstract: Flavourzyme and trypsin were used in combination for one-step hydrolysis of oyster homogenate. The resulting hydrolysate was separated by Sephadex G-25 column chromatography. As a result, a bioactive peptide was obtained. The apoptosis inducing effect of the bioactive peptide on HeLa cells was examined by sulforhodamine B colorimetric assay, trypan blue staining assay, Annexin V-FITC/PI double staining assay and DNA ladder assay. Meanwhile, the mechanism was analyzed by flow cytometry and real-time PCR. This peptide had a molecular weight of 751 D. It could effectively inhibit the proliferation of HeLa cells, arrest the cell cycle transition from G1 to S phase and induce the up-regulation of apoptotic gene expression. Its mechanism of action was probably associated with up-regulating apoptotic gene expression and arresting the cell cycle.

Key words: HeLa cell, bioactive peptide from oyster, apoptosis, cell cycle arrest

CLC Number:

Q2544

References

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