Abstract:

In order to exploit new carbon and nitrogen sources for Hericium erinaceus, effects of kind and amount of carbon and nitrogen sources on the content of polysaccharide in harvested mycelia and broth of Hericium erinaceus after 35-day liquid submerged fermentation. The phenol-sulfuric acid method was used to determine polysaccharide content. Results showed that the optimal carbon and nitrogen sources were starch and soybean dregs for polysaccharide production in Hericium erinaceus mycelia, respectively, and the content of polysaccharide was as high as 162.17 mg/g when the amounts of starch and soybean dregs were 4.0% and 1.5%. In conclusion, starch and soybean dregs are the optimal choices of carbon and nitrogen sources for polysaccharide production in Hericium erinaceus due to low cost and broad source, which will have a promising popularilization prospect in practical production.

Key words: Hericium erinaceus, polysaccharide, liquid submerged culture, phenol-sulfuric acid method