Abstract:

The objective of the present work was to study the effects of polysaccharides from Chinese rice wine on immunityrelated
cytokines in immunodeficient mice induced by cyclophosphamide (CY). After fifty mice were fed adaptively for
four days, they were divided into five groups: blank control, cyclophosphamide, cyclophosphamide + polysaccharides
from Chinese rice wine (50, 100 and 200 mg/(kg·d)). The mice in CRWP + CY (50, 100 and 200 mg/(kg·d)) groups were
administered with polysaccharides from Chinese rice wine by gavage once a day for 14 days, and the mice from the blank
control group and CY group were fed with the same volume of physical saline. Then, the mice in CY group and CRWP +
CY group were injected intraperitoneally with CY (100 mg/(kg·d)) once a day for 4 days. On the 22th day, the mice were
executed and the blood was collected from the retro-orbital plexus and centrifuged for analysis. Interleukin 6, interferon-γ
tumor necrosis factor-α, immunoglobulin A, immunoglobulin M, immunoglobulin G and complement C3, complement
C4 in mouse serum were measured by ELISA. Compared with the controls, interleukin 6, interferon-γ, tumor necrosis
factor-α, immunoglobulin A, immunoglobulin M, immunoglobulin G and complement C3, complement C4 in mouse serum
were increased. The level of interferon-γ in CRWP + CY (200 mg/(kg·d)) group was significantly higher than that in the
blank control group. These results indicate that polysaccharides from Chinese rice wine can enhance immune function in
immunodeficient mice especially at higher dose.

Key words: polysaccharides from Chinese rice wine, immunodeficient mice, cyclophosphamide, immunocompetence