Antimutageic activity of casein and its pepsin hydrolysis product were studied with Salmonella Ames test.The digestion products have Shown stronger antimutagenicity against sodium azide than casein.The hydrolyzed peptides formed were separated by ultrafiltration or isoelectroic precipitation,and were characterized by FPLC and Mass spectra analysis.The peptides molecular weigh range was between to 1000 to 2000 dalton.and contained 9 to 11 amino acids.

Some physical and chemical properties of polysaccharides of Agaricus blazei were investigated in this paper. The results showed that three groups of purified polysaccharide were free from urinic acid. The protein content of the polysaccharides was AB-I 20.1%, AB-II-b 16.3% and AB-III-b 25.6%. The specific rotatory power of the polysaccharides was AB-I +44.33°, AB-II-b +3.70°, AB-III-b +5.04°. The viscosity of AB-I and AB-II-b was increased with the increase of solution concentration . The viscosity of 1% AB-I and AB-II-b was stable in different concentration of NaCl, pH value and temperature.

Measurements of age,carcass weight,deboned beef weight,beef cus weight,cut weight and others were made in this experiment by slaughtering 82 non-fattened yellow cattle.Correlation coeficients between above indices and regression equations applicable to predict carcass yield were established.Meanwhile,palatability of beef 24h postmortem was studied The results showed that different cut were different in shear force value,tenderness and juiciness.The sensory panel score was inconsistent with the shear force value.

The three flour samples were analyzed,which have been comminuted and classified by the CH-300 mechanic impact instrument designed by ourselves.The results showed that the average powder diameter of the finest flour comminuted was 5.98μm and powder shape approximated to sphere symmetry.The flour protein content increased as powder getting finer.It became two times more as power diameter D90 being smaller than 10μm ,thus providing a new way for high protein flour blending.

In this paper, ozone water was prepared by directly dissolving ozone gas in water, and its concentration was measured by ultraviolet absorption method corrected by iodine stoichiometry titration method.By chemical kinetics method, the effects of water quality, temperature and pH value on the stability of ozone water were studied, and a preliminary approach about stabilizing ozone in aqueous solution with acetic acid, citric acid and so on was provided in this paper. The experimental results showed that water quality, temperature and pH value were important factors affecting the stability of ozone water. The better water quality was, the more stable ozone water was, and ozone decomposed very slowly in double-distilled water. Lowering water temperature could enhance the stability of ozone water. Ozone decomposed much quickly in alkaline solution, and its decomposition was heavily inhibited in acid solution, but excessive acidity could also accelerate ozone’s decay and reduce the stability of ozone water. Additionally, a little acetic acid or citric acid could greatly improve the stability of ozone water. As far as the effect was concerned, acetic acid was better than citric acid. Sodium chloride could hardly increase the stability of ozone water, and it accelerated ozone’s decay when a large amount was added.

The AOT-n-haxane-KCl as the reverse micelle solution, used to extract the protein and oil from soybean was studied. The extraction and back-extraction of the protein was tested in various conditions such as the rate of soybean weight against the reverse micelle solution volume,mixed-time,temperature,the pH value of aqueous and the salt oncentrations.On this basis,the conditions fit to the extraction and back-extraction from soybean with AOT-n-haxane-KCl reverse micelle were determined.The results in dicated that the highest extraction rate of the protein was 96.9percent and the soybean oil was 90.5 percent.

Sieving culturers showed Lp and La could live under the 6% of NaCl and 1.5×10-4of NaNO2;Lp was better than La in producing acid and resisting NaCl and NaNO2;Two culturirs Coud not affect the decomposition of protein and fat.

The study on isolation and property of exopolysaccharides from Armillaria mellea was conducted.The following results were obtained : The fermentation broth was concentrated. Then alcohol was put thrice into it .The precipitate attained was eluted again with 75% alcohol .The protein was eliminated with Sevage method.Furthermore,the DEAE-cellulose column chromatography was used on the exopolysaccharide purification and the column was eluted with the distilled water and NaCl solution from 0.05～0.5mol/L.Neutral and several kinds of acid polysaccharides were obtained . The purification of the neutral polysaccharide was made with SephadexG-150 chromatography.The pure polysaccharide A was collected. The IR spectrum of the polysaccharide A showed a character of β-glycosidic bond in 400～4000cm-1.After the polysaccharide A was hydrolyzed, the hydrolysate was analyzed with the silica gel pellicular chromatography and filter paper chromatography . While both the chloroform:methanol(60:40) and acetone:H2O(96:4) were used as the disperse agent and the dische reaction was used as dyestuff, the results proved that polysaccharide was composed of glucose. The polysaccharide was colored by coomassie brilliant blue G-250, and coomassie brilliant blue R-250 after polysaccharide electrophoresis. It proved that there was not any protein remained in the polysaccharide A.

Artemis sphaerocephala kraschen’s gum was tested by concentric cylinder viscometer. The result showed that A.S.kraschen’s gum was Thixotropic pseudroplastic(time-dependent fluid) at 1%～1.75%, and pseudroplastic(time-independent fluid) at 0.5%. Temperature and pH had no effect on viscosity of the gum fluids.

Nitrite isConsidered considered harmful to people’s health .In this paper the forming of nitrite in the process of pickling of potherb mustard has been studied in detail. Through experiments the relations between the forming of nitrite and several external factors have been found. The results suggested that: (1) the speed of forming and content of nitrite were related to the concentration of sodium chloride; (2) the temperature of fermentation affected the speed of the forming and content of nitrite; (3)acid environment was unfavourable to the forming of nitrite ;(4)glucose was favourable to reduce the content of nitrite in the potherb mustard; (5)pollution of microorganism might increase the content of nitrite.

The ginger juice Could make pig meat tender by the following conditions:the concentration of ginger juice5%,pH value7.0 and temperature40℃.

In order to establish the quantitative determination method for C21 steroidal glycosides in Asclepiadaceae plants and homologous health food,the extraction methods of C21 steroidal glycosides were investigated fist in this paper.The reaction of 2-desoxy sugar for cardiac glycosides’quantitative determination by thin layer chromatogaphy was used as a relative quantitative determination method for the comparison of all kinds of extraction methods.Accorfing to the structure of C21 steroidal glycosides,11 kinds of extractes were selected to do extraction experiments.The effects of other factors Such as mesh size of the material and the extraction time were also studied. At last the extraction method of C21 steroidal glycosides was determined.

In this paper, the isolation and identification of Yoghurt culture bought from the market were studied.The result of the experiment showed: three bacteria strains from the Culture were isolated, where one was Lactobacillus bulgaricus and the others were Streptococcus thermophilus.They have Showed strong effect on the production of acid in milk. Using them to produce Yoghurt, the optimum proportion of culture and milk was 4%and the ratio of coccus and bacillus was 2 :2 in composite fermentation. The quality of the product was superior .

Chlorella is a kind of edible alga with high protein content and chlorella growth factor.Chlorella cell wall was availably broken open through rehydration at 60℃ and homogenization at 30MPa,and the cell protein extraction ratio was improved to some extent by the enzymic degradation with cellulase and pectic enzyme.With the further degradation process by basic proteinase and neutral proteinase(30,000U/30,000U per gram of dry chlorella powder) at pH8.0、65℃ for 4 h,the chlorella extraction was converted into stable solution . Finally a clarified chlorella health beverage was prepared with the deodorized and filtrated solution.

Factors influencing the quality of the honey yoghourt were studied. The changes of pH and acidity in the preserve test were measured.And the chemical and physical properties of the product were analyzed. Through orthogonal test, we got the optimal conditions of making high quality honey yoghourt: proportion between Streptococcus therophilus and Lactobacillus bulgaricus was 1, the inoculation was 1%, the additive of agar was 0.2%,and the fermentation temperature was 42℃.The product could be stored below 10℃ for 24 with no obvious change of pH and acidity in the product . The result of sensory check and chemical and physical analysis showed that a better quality would be gained if we use honey as a modification and alternative of sugar in the yoghourt fermentation.

The article mainly studied the application of PAAS in the ice cream product.By the single factor experiment and many factors vertical experiment,we could find: the reduction ofpH and the reduction of the viscosity of PAAS.The affiliation of Sudium Chloride could lower the viscosity of PAAS:the water fitting the normal drink had no effect on the viscosity of PAAS.Under the temperature of sterilization,the longer the sterilization, the lower the viscosity .By the mulif factor vertical experiment,we could get that the optimum experiment formula is: percentage of treacle,mixture of guar gum,Konjac gum:carrageenan(=4:1)and PAAS was 60%,0.55%, 0.15%and 0.2%respectively.

In this paper,the flavor compositions of the watermelon were analyzed.It has introduced in detail the not able composition of watermelon flavors on the basis of the characteristic odor. The experiment of compounding made by a variety of aromachemicals selectively has shown the reasonable compositions of ingredients.

A new simple but effective method for breakingGanoderma-lucidum spore by quenching into subcooled liquid nitrogen was studied in this paper.As the result of the experiment,the breaking ratio increased with the increase of freezing-defreezing times.

Chloro is ocean vegetable with values of medicament and nutrition.This paper chiefly discussed process prescription and main technique points of the chloro-fruit juice compound drink by using Enteromorpha prolifera J.Ag.as main raw material.Meanwhile,it interpreted the ways of getting rid of the Chloro’s fishodor,and the product quality standard as well.

The Chicken which was just born was used as raw material in the experiment to produce chicken sofe can.It was handled by these techniques ,Such as butchering,pickling drying formenting and tosting.The product showed low calorie,plentiful nutrient contents and favorable taste.

DSC method was found to be a simple , rapid and precise method appied to purity determination. Lycopene crystal was preparaed. Its purity was determined with DSC. The analysis result was compared with HPLC and UV-Visible methods.

This article introduced a new method to determine free SO2 in red wine—photometric titration.It was quick and simple, with good accurate and high precision.The automation of operation could be easily achieved. It was very suitable for the analyzing trace of free SO2 during the course of winemaking.

A method using pre-column derivatization with o-phthaldialdehyde(OPA) and fluorenylmethyl chloroformate(FOMC chloride)was reported for the determination of amino acids in sample by RP-HPLC.The analysis could be accomplished within 17.0 min by using Hypersil C18. RP-HPLC followed by FLD detection. The method possesses the advantages of effective separation, good reproducibility and satisfactory linear range.

The color reaction between Pb2+and CPA-Ⅲ was studied. In the medium of 0.2mol/L HCl, lead could form a violet-red complex with CPA-Ⅲ. The maximum absorption of the complex occurred at 650nm .The apparent molar absorptivity was 5.7×104L•mol-1•cm-1, and Beer’s law was obeyed in the range of 0～15μg(Pb)/25ml. This method has been applied to determinate trace lead in cosmetics and water successfully.

A new method was described for the determination of copper, iron and zinc in maize flour by micro-suspension sample introduction derivative flame atomic absorption spectrometry. The optimum conditions concerning trace elements the relative sensitivity the accuracy and the detection limits were 0.019μg/ml, 0.058μg/ml and 0.013μg/ml, respectively. This method was sensitive, accurate, precise and rapid.

This method was based on the SeSO32--KIO3 principle of oxidation and reduction.Through using 273A type Electric Chemical Analytic System(Made in America,1992),the trace selenium of selenium-rich rice was determined by the method of differential pulse catalytic polarography.

To strengthen management of healthy and safe meat,we take 58 samples from fresh and frozen beef to be sold in shenzhen city .These samples were assayed according to domestic standard of inspection.The results showed that the fresh and frozen beef to be sold in shenzhen was not contaminated with 0157:H7E.coli.

Effects of modified atmosphere packaging and ethylene absorbent treatment on physiology and green preservation in Green Mume were investigated at 25℃。The results indicated that in both modified atmosphere packating and ethylene absorbent treatment,the rate of respiration and production of enthylene slowed down and the contents of chlorphyll and ASA also reduced while fruit firmness was relatively maintained.The treatment of modified atmosphere packaging was better than that of ethylene absorbent.Both treatments improved SOD activity and inhibited the POD activity and the O2-.production rate as well in Green Mume fruits.It suggested that both treatments delayed the degradation of chlorophyll through maintaining the balance of activated oxygen metabolism and inhibiting the rate of respiration and production of enthylene in Green Mume fruits.

The effect of 1-methylcyclopropene (1-MCP) to keep peach fruits fresh was studied. Peach fruits were fumigated for 24 h at 5 ℃ with 1-MCP at 500nL/L and then stored for 28 days at 5 ℃. The results showed that the browning rate of peach flesh treated by 1-MCP were depressed largely. The flesh browning indices of peach treated by 1-MCP were 31%, however, those of control were 62%. 1-MCP as a new gaseous inhibitor of ethylene action will be available for inhibiting the flesh browning of peaches in future.

Pests and germination of Chinese chestnuts may be controlled effectively with 60Coγ-ray irradiation . The suitable doses of irradiation for pest and germination were 800Gys and 250Gys respectively . The low doses of irradiation were good for preservation of chinese chestnuts, and the middle-high doses of irradiation were bad for preservation chinese chestnuts.

yr studies revealed that CA storage with 1%O2+5%CO2at 3℃was not favorable for fruit of peach Feicheng, because 3℃ and 1%O2 caused generation of flesh browning earlier than 0℃ and 5%O2.CA storage with 5%O2+5%CO2 at 0℃in combination with intermittent raising of temperature 5%O2 +5%CO2 significantly delayed the beginning of flesh browning, better maintained fruit firmness, and prolonged the storage period up to 70d with shelf life being of 5d, keeping the fruit in good consuming quality.

dipheny1-1,3,5-hexatriene,a kind of fluorescence probe,was used to observe the effects of cole pollen flavonoids on the oxidative damage in rat erythrocyte ghost induced by peroxy radical and hydroxy radical.The results demonstrated that both peroxy radical and hrdroxy radical increased the content of malondialdehyde(MDA),decreased membrance fluidity significantly,and caused lipid peroxidation of rat erythrocyte ghost.But cole pollen flavonoids could decrease the content of MDA remarkably,improve the membrance fluidity of rat erythrocyte membrance fluidity and inhibit the lipid peroxidation in rat erythrocyte membrance .These results suggested that cole pollen was a kind of natural antioxidant and could prevent lipid peroxidation in rat erythrocyte membrance damage induced by peroxy radical and hydroxy radical.

The antimicrobial activities of EGb (Extract of Ginkgo biloba) against common food contaminated microorganisms were determinded. The effects of heating conditions and pH value on the antimicrobial activities were discussed. The antimicrobial characteristics of EGb and the protection for the development of EGb as a natural food antiseptic were envisaged. Results showed that the antimicrobial activity of EGb was high and increased with the increase in the concentration of EGb, and the minimal inhibition concentrations(MIC) were 1.25% against bacteria, and 5.0% against fungi. And the antimicrobial activity of EGb was characterized by its heat stable ability, and high temperature was not effective to its antimicrobial activity. The antimicrobial activity pH value ranged from 5 to 9. This research provided experimental basis for development of EGb as a natural food antiseptic.Key words Extract of Ginkgo biloba Antimicrobial activity Heat stable ability pH value Natural food antiseptic

This paper determined the 55 chemistry element contents by ICP-MS、AAS and others of Sauropus androgynus L,which grew in Hainan Island.It showd that there was the likelinood to repel cancer because of the rich content of the Zn、Mn.Compared with vegetable in the contents of elements needed by human beings,Sauropus androgynus L.had lower Na ,but their contens were almost several times,more in K、Ca、Mg、Fe、Zn、Mn、Cu、P and protein.

The health food-Sedum Scrmentosum Bunge liver protection capsule was made with Sedum Scrmentosam Bunge and Spirulina platensis as raw materials .We established the relative quality standard and made the safety assessment and function evaluation of the capsules. Rusult: 1.The acute toxicity of the capsules belonged to non-toxicity. The capsules had no mutagenic actions. No obvious symptoms were observed in rats after 30 days oral administration. The no effect level was 5.0g/kg body weight. 2. The capsules had the protect effect for mice liver injury. The ALT in serum in low and moderate dose group was both lower than those of control group (p<0.05).The liver pathological injury in each group was more alleviated than those in control group - the CCl4 group.

The objects were Kunming mice (♂). The model was set up by continuous administration of MT during 51 days. The indexes included the content of Hemoglobin(Hb) and Malondialdehyde(MDA), the activity of Glutamic-pyruvic Transaminase in serum(SGPT), Glutamin-oxalaceticTransaminase(SGOT) and Superoxide Dismutase(SOD) and Total anti -oxidant Capability(T-AOC). The results showed that continuous administration of MT did not significantly affect Hb content, the activity of SOD in liver and SGOT(P>0.05).The activity of SGPT and the content of MDA in liver and serum significantly decreased(P<0.01). But total anti-oxidation capability(T-AOC)significantly rose(P<0.05). Experimental results suggested that MT being strong in anti-oxidation capability could significantly assist anti-oxidation system of the body.

Aloe Polysaccharide was purified by DEAE-Cellulose and SephendexG-200 sequentially.A pure neutral polysaccharide AP-A-2 was gotten.It was proven by the test that AP-A-2 shows no evident effect in scavenging . andH2O2but only a little effective in scavenging •OH,but Ap-A-2 showed no evident effect in scavenging R• or ROO•.

To study the health functions of crude extracts from squid spermary tissues.Experiments for evaluating the health functions including anti-fatigue effect,anti-oxidization effect and immuno-regulation activity of crude extracts from squid spermary tissues were performed in this study.It showed that the in mice investigated extracs could lengthen the time of swimming and rodding,decrease the consumption of live glucogen during sporiting and lower the level of blood lactic acid after sport.It also revealed that the crude extraction could lower the level of blood malondialdehyde (MDA) and heighten the SOD activity in blood,while at the same time raise the NK cell activity,macrophage rate,phagocytosis index and leves of cell immunology.The investigated extracts had effects of anti-fatigue,anti-osidization and immuno-regulation and thus demonstrated the potential for being developed into a natural marine health food.

We extracted the components from the branch,leaf ad fruit of Fig by different methods,and determined the contents of benzaldehyde (UV dual-wave),Selenium(Se,colorimetry),sugar(HPLC),and protein(Kjeldahl’s method )in different extracts.The results showed that the benzaldehyde and Se were highest in leaf;protein was highest in water extract of all parts;and sugar content was related with its molecular weight and different in fig.These suggested that all of the branch,leaf and fruit of fig have had nutritive and pharmaceutical values.

With the result of rapidly increasing number of completed and ongoing genome sequences of food-related microbes,comparative and functional genomics approaches are being developed for employing these data in the benefit of the food biotechnology.These developments not only offer a better understanding of microbial cell physiology and its metabolic function but also provide mant possibilities to improve the functional properties of foods by Utilizying food grade microorganisms and to increasc the safety of foods as well.

Molecular imprinting has come a long way since its modest beginnings more than sixty years ago.Within latest years in particular,the research interests in the area have surged.The development potential of MIP technology is high,since MIPs have showed physical robustness,high mechanical strength and stability in extremes of environment.More importantly,the MIPs have exhibited high selectivity and recognized activity to the template(the analyte).In this article,recent research of MIP technology was reviewed,details of preparation,application in separation、assays and sensors、biological molecular mimics and catalyst were introduced.Although the technique faced challenge in some aspects it has revealed enormous potential value in marketing.

Human Milk Oligosaccharides (HMO)is a sort of natural oligosaccharides existing in human milk.They not only have protective functions against intestinal infections but also play an important role in keeping gastrointestinal ecosystem balance. In addition,they also have many other important biological functions under investigation.In the struggle of human being against pathological microorganisms,HMO offers us a new concept and a valuable choice.

The research history on food irradiation in Japan was reviewed and most of the recent last reseach achievements were introduced in this paper.Especially,studies achievements on identification methods of irradiated food,evaluatio of induced radioactivity in irradiated food,the flavor changes in irradiated food and mutagenesis of irradiated food in Japan were presented.

According to the demands on the food cold storage by the refrigeration technology,the computer was used to control the food cold storage.The control system design was discussed briefly.

Phytoestrogens,such as isoflavones,lignans and coumestrols were natural bioactive constituents of soybean,sesame,rice,fruits and vegetables and exhibited vital biological functions for human health.These compounds have received increasing attention because of their interesting biological properties and pharmacological role in prevention of human cancer and other diseases.The present study reviewed the newdevelopments on analysis method,relationships between structure and function as well as prevention of diseases,especially cancer and coronary heart diseases.The author also suggested the development prospect of functional foods by using phytoestrogens as biofactor.

In this paper the present devdelopment of membrane separation technology in beer industry was reviewed.The application and piogress of membrane separation technology of MF,UF,RO,DA,GS,etc,in asepsis filtration ,free alcohol beer making,fresh beer making,recovery beer from yeast,deoxygenation in diluent water,getting nitrogen from air etc were elucidared Due to high efficiency,low energy,no waste,low invest,membrane separation technology showed a great prospect in promoting the taste,quality and output of beer.

A new research field about ice nucleation active (INA) bacteria and INA protein which is applied to many fields including food engineering has been studied in the world,since INA bacteria was detected by L.R.Maki in 1974. Studies of the safe application of INA bacteria is attached more and more importance to for the special demand of bacterium of food industry. A lot of techniques emerged gradually, such as high pressure sterilization, purification of INA protein, immobilization, INA protein power, radialization and transgene. This review reports the progress on the technology of the safe application of INA bacteria.